The retinoblastoma (RB) gene was the first tumor suppressor gene identified. It encodesa nuclear phosphoprotein which is differentially phosphoryIated during the cell cycle. And the RB geneapparently plays a key role ...The retinoblastoma (RB) gene was the first tumor suppressor gene identified. It encodesa nuclear phosphoprotein which is differentially phosphoryIated during the cell cycle. And the RB geneapparently plays a key role in cell growth regulation. Mutations in RB are seen in virtual1y all cases ofrehaoblastoma, and loss of RB gene function has been implicated in the progression of many common humancancers. A number of studies have indicated that replaccment of thc normal RB genc in Rl3-defective tumorcells could suppress their tumorigenic activity in nude mice. Preclinical studies also demonstrated that treatmentof established human xenografttumors in nude mice by recombinant adenovirus vectors expressing RB proteinresulted in regression of the treated tumor . These studies make the emerging RB gene therapy even more attraction.展开更多
This study was supported in part by grant from National Natural Science Foundation of China (No. 39570775). Objective To investigate the vascular smooth muscle cell (SMC) growth suppression by recombinant adenovir...This study was supported in part by grant from National Natural Science Foundation of China (No. 39570775). Objective To investigate the vascular smooth muscle cell (SMC) growth suppression by recombinant adenovirus vector expressing a retinoblastoma (Rb) protein and to explore a gene therapy approach for vascular proliferative disorders including atherosclerosis and artery restenosis. Methods A replication deficient adenovirus vector encoding a wild type Rb and AdCMVRb, was constructed and transfected into cultured rabbit aortic SMC. The efficiency of gene transfection and expression was detected by immunochemical staining and polymerase chain reaction. The role of Rb in regulating vascular SMC proliferation was observed by cell counting, thymidine incorporation, and flow cytometry. Results Wild type Rb gene transfected effectively into the cultured SMC with AdCMVRb can suppress growth factor stimulated cell proliferation through regulation of DNA synthesis and cell cycle progression. Conclusion The results demonstrate the potential of adenovirus mediated Rb gene therapy for atherosclerosis and artery restenosis after balloon angioplasty.展开更多
Objective: To study the growth suppression of lung adenocarcinoma cell by the introduction of wild type P53 gene and explore a gene therapy approach for lung adenocarcinoma. Methods: A replication deficient adeno v...Objective: To study the growth suppression of lung adenocarcinoma cell by the introduction of wild type P53 gene and explore a gene therapy approach for lung adenocarcinoma. Methods: A replication deficient adeno virus vector encoding a wild type P53 was constructed and transfected into the cultured human lung adeno carcinoma cell line GLC 82. The efficiency of gene transfection and expression was detected by immuno chemical staining and polymerase chain reaction. The cell growth rate and cell cycle were analysed by cell counting and flow cytometry. Results: Wild type P53 gene could be quickly and effectively transfected into the cells by adenovirus vector. Wild type P53 expression could inhibit GLC 82 cell proliferation and induce apoptosis. Conclusion: The results indicated that recombinant adenovirus expressing wild type P53 might be useful vector for gene therapy of human lung adenocarcinoma.展开更多
The molecular genetics of the mitochondrial genome has become a subject of rapidly increasing interest. But study on the mitochondrial genome of bird is rather rare. We have purified the mitochondrial DNA (mtDNA) from...The molecular genetics of the mitochondrial genome has become a subject of rapidly increasing interest. But study on the mitochondrial genome of bird is rather rare. We have purified the mitochondrial DNA (mtDNA) from the liver of Peking white duck and studied its physical structure, including the estimation of the contour length and the molecular weight as well as the construction of the physical map.展开更多
Objective: To study the role of the most extensively studied tumor suppressor gene, retinoblastoma (Rb) gene, on the growth of lung adenocarcinoma cell line GLC 82 and explore a gene therapy approach for lung adenoca...Objective: To study the role of the most extensively studied tumor suppressor gene, retinoblastoma (Rb) gene, on the growth of lung adenocarcinoma cell line GLC 82 and explore a gene therapy approach for lung adenocarcinoma Methods: The recombinant Rb gene adenovirus vector was constructed, the control virus which carries LacZ gene was producted by the same method Infection effects were detected by biochemical staining of β gal and immunohistochemical analysis of Rb protein The Rb cDNA of infected cells were determined by PCR The cell growth rate and cell cycle were observed by cell counting and flow cytometry Results: The constructed recombinant adenovirus vector could infect effectively the cells with high level expression of Rb cDNA and Rb protein The transfection of wild type Rb gene could suppress GLC 82 cell proliferation and decrease the cellular DNA synthesis Conclusions: These results showed the possibility of using recombinant Rb gene adenovirus vector in the gene therapy of cancer to inhibit the growth of cancer展开更多
Researches on oncogenes from human malignant tumors have terminated the long history in which mankind groped and hesitated in the dark to conquer cancer and have become the milestone of the modern molecular oncology. ...Researches on oncogenes from human malignant tumors have terminated the long history in which mankind groped and hesitated in the dark to conquer cancer and have become the milestone of the modern molecular oncology. An established genomic library from a solid tumor or a tumor cell line could be screened by sib-selection to search for unknown oncogenes and even antioncogenes (tumor suppressors). Furthermore,展开更多
文摘The retinoblastoma (RB) gene was the first tumor suppressor gene identified. It encodesa nuclear phosphoprotein which is differentially phosphoryIated during the cell cycle. And the RB geneapparently plays a key role in cell growth regulation. Mutations in RB are seen in virtual1y all cases ofrehaoblastoma, and loss of RB gene function has been implicated in the progression of many common humancancers. A number of studies have indicated that replaccment of thc normal RB genc in Rl3-defective tumorcells could suppress their tumorigenic activity in nude mice. Preclinical studies also demonstrated that treatmentof established human xenografttumors in nude mice by recombinant adenovirus vectors expressing RB proteinresulted in regression of the treated tumor . These studies make the emerging RB gene therapy even more attraction.
文摘This study was supported in part by grant from National Natural Science Foundation of China (No. 39570775). Objective To investigate the vascular smooth muscle cell (SMC) growth suppression by recombinant adenovirus vector expressing a retinoblastoma (Rb) protein and to explore a gene therapy approach for vascular proliferative disorders including atherosclerosis and artery restenosis. Methods A replication deficient adenovirus vector encoding a wild type Rb and AdCMVRb, was constructed and transfected into cultured rabbit aortic SMC. The efficiency of gene transfection and expression was detected by immunochemical staining and polymerase chain reaction. The role of Rb in regulating vascular SMC proliferation was observed by cell counting, thymidine incorporation, and flow cytometry. Results Wild type Rb gene transfected effectively into the cultured SMC with AdCMVRb can suppress growth factor stimulated cell proliferation through regulation of DNA synthesis and cell cycle progression. Conclusion The results demonstrate the potential of adenovirus mediated Rb gene therapy for atherosclerosis and artery restenosis after balloon angioplasty.
文摘Objective: To study the growth suppression of lung adenocarcinoma cell by the introduction of wild type P53 gene and explore a gene therapy approach for lung adenocarcinoma. Methods: A replication deficient adeno virus vector encoding a wild type P53 was constructed and transfected into the cultured human lung adeno carcinoma cell line GLC 82. The efficiency of gene transfection and expression was detected by immuno chemical staining and polymerase chain reaction. The cell growth rate and cell cycle were analysed by cell counting and flow cytometry. Results: Wild type P53 gene could be quickly and effectively transfected into the cells by adenovirus vector. Wild type P53 expression could inhibit GLC 82 cell proliferation and induce apoptosis. Conclusion: The results indicated that recombinant adenovirus expressing wild type P53 might be useful vector for gene therapy of human lung adenocarcinoma.
文摘The molecular genetics of the mitochondrial genome has become a subject of rapidly increasing interest. But study on the mitochondrial genome of bird is rather rare. We have purified the mitochondrial DNA (mtDNA) from the liver of Peking white duck and studied its physical structure, including the estimation of the contour length and the molecular weight as well as the construction of the physical map.
文摘Objective: To study the role of the most extensively studied tumor suppressor gene, retinoblastoma (Rb) gene, on the growth of lung adenocarcinoma cell line GLC 82 and explore a gene therapy approach for lung adenocarcinoma Methods: The recombinant Rb gene adenovirus vector was constructed, the control virus which carries LacZ gene was producted by the same method Infection effects were detected by biochemical staining of β gal and immunohistochemical analysis of Rb protein The Rb cDNA of infected cells were determined by PCR The cell growth rate and cell cycle were observed by cell counting and flow cytometry Results: The constructed recombinant adenovirus vector could infect effectively the cells with high level expression of Rb cDNA and Rb protein The transfection of wild type Rb gene could suppress GLC 82 cell proliferation and decrease the cellular DNA synthesis Conclusions: These results showed the possibility of using recombinant Rb gene adenovirus vector in the gene therapy of cancer to inhibit the growth of cancer
文摘Researches on oncogenes from human malignant tumors have terminated the long history in which mankind groped and hesitated in the dark to conquer cancer and have become the milestone of the modern molecular oncology. An established genomic library from a solid tumor or a tumor cell line could be screened by sib-selection to search for unknown oncogenes and even antioncogenes (tumor suppressors). Furthermore,
