Dependence of surface-enhanced Raman scattering （SERS） from Calf thymus DNA on anions is investigated. With the silver colloid, the bands at 732, 960 and 1333 cm^-1 for adenine （A）, 1466 cm^-1 for deoxyribose, and...Dependence of surface-enhanced Raman scattering （SERS） from Calf thymus DNA on anions is investigated. With the silver colloid, the bands at 732, 960 and 1333 cm^-1 for adenine （A）, 1466 cm^-1 for deoxyribose, and 1652 cm^-1 for the C=O group of thymine （T） are observably enhanced. With the presence of the Cl^- or SO4^2- anions, the bands at 732 and 1326/1329 cm^-1 for the symmetric stretching and skeletal vibrational modes of adenine （A） are dramatically enhanced, and the enhancement effect with the SO4^2- ion is more than that with the Cl^- ion. The experimental results show that the DNA molecule can be adsorbed on the silver colloid particles through the C6N and N7 of adenine （A） the C=O of thymine （T） and deoxyribose. Moreover, the formed hydrogen bonding of the Cl^- or SO4^2- ions to the C6NH2 group of adenine （A） can induce larger C6N electronegativity, which is favor for the C6N/N7 cooperative adsorption on the （Ag）^＋ colloid particles.展开更多
基金the National Natural Science Foundation of China (No.10574046)the National Key Project for Basic Research of China (No.2006CB806006 and 2006CB921105)+3 种基金the Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT)the Program for New Century Excellent Talents in University (NCET-04-0420)the Doctoral Program of High Education (No.20050269011)and the Phosphor Program sponsored by Shanghai Science and Technology Committee (No.06QH14003).
文摘Dependence of surface-enhanced Raman scattering （SERS） from Calf thymus DNA on anions is investigated. With the silver colloid, the bands at 732, 960 and 1333 cm^-1 for adenine （A）, 1466 cm^-1 for deoxyribose, and 1652 cm^-1 for the C=O group of thymine （T） are observably enhanced. With the presence of the Cl^- or SO4^2- anions, the bands at 732 and 1326/1329 cm^-1 for the symmetric stretching and skeletal vibrational modes of adenine （A） are dramatically enhanced, and the enhancement effect with the SO4^2- ion is more than that with the Cl^- ion. The experimental results show that the DNA molecule can be adsorbed on the silver colloid particles through the C6N and N7 of adenine （A） the C=O of thymine （T） and deoxyribose. Moreover, the formed hydrogen bonding of the Cl^- or SO4^2- ions to the C6NH2 group of adenine （A） can induce larger C6N electronegativity, which is favor for the C6N/N7 cooperative adsorption on the （Ag）^＋ colloid particles.
