通过主成因判别分析法(discriminant analysis of principal component,DAPC)建立仿刺参个体水平地理亚群体来源的判别模型。实验运用了20个微卫星引物,对俄罗斯海参崴(RS)和中国大连两个亚群(CN1、CN2)3个仿刺参地理群体进行了产物扩...通过主成因判别分析法(discriminant analysis of principal component,DAPC)建立仿刺参个体水平地理亚群体来源的判别模型。实验运用了20个微卫星引物,对俄罗斯海参崴(RS)和中国大连两个亚群(CN1、CN2)3个仿刺参地理群体进行了产物扩增和标记分型,获得3个地理群体分子标记表型数据。开创性地运用主成因分析和判别分析法建立判别模型,以研究不同群体仿刺参个体水平上遗传背景及其群体来源。结果显示,运用20对微卫星引物在仿刺参不同地理群体中稳定扩增的136个等位基因位点数据建立的判别模型可以准确判别刺参个体的群体来源。用75%的原有数据集建立模型,剩余的25%数据对模型的准确度进行验证,3个群体时模型准确率大于80%,2个群体时模型准确性超过90%。传统的遗传多样性和遗传结构分析显示,刺参的3个地理群体间存在中度的遗传分化,且遗传距离较近(D1=0.18,D2=0.159)。传统分析方法和新方法结果良好的一致性表明,利用DAPC和微卫星等位基因信息建立的刺参地理群体判别模型,可以很好地用于未知遗传背景刺参种质的来源鉴别。展开更多
Environmental conditions, including ambient temperature, play important roles in survival growth development, and reproduction of the Japanese sea cucumber, Apostichopus japonicus. Low temperatures result in slowed gr...Environmental conditions, including ambient temperature, play important roles in survival growth development, and reproduction of the Japanese sea cucumber, Apostichopus japonicus. Low temperatures result in slowed growth and skin ulceration disease. In a previous study, we investigated the effect of low temperature on gene expression profiles inA.japonicus by suppression subtractive hybridization （SSH）. Genes encoding Ferritin, Lysozyme, Hsp70, gp96, and AjToll were selected from a subtracted cDNA library of A. japonicus under acute cold stress. The transcriptional expression profiles of these genes were investigated in different tissues （coelomocyte, respiratory tree, intestine, longitudinal muscle） after exposure to acute and mild temperature dropping treatments. The results show that （1） the five cold-tolerance-related genes were found in all four tissues and the highest mRNA levels were observed in coelomocyte and respiratory tree; （2） under the temperature dropping treatments, three types of transcriptional regulation patterns were observed： primary suppression followed by up-regulation at -2℃, suppressed expression throughout the two treatments, and more rarely an initial stimulation followed by suppression; and （3） gene expression suppression was more severe under acute temperature dropping than under mild temperature dropping treatment. The five cold-tolerance-related genes that were ,distributed mainly in coelomocyte and respiratory tissues were generally down-regulated by low temperature stress but an inverse up-regulation event was found at the extreme temperature （-2℃）.展开更多
Fully grown oocytes of Apostichopus japonicus have a cytoplasmic protuberance where the oocyte attaches to the follicle. The protuberance and the oolamina located on the opposite side of the oocyte indicate the animal...Fully grown oocytes of Apostichopus japonicus have a cytoplasmic protuberance where the oocyte attaches to the follicle. The protuberance and the oolamina located on the opposite side of the oocyte indicate the animal-vegetal axis. Two pre-meiotic centrosomes are anchored to the protuberance by microtubules between centrosomes and protuberance. After meiosis reinitiation induced by DTT solution, the germinal vesicle (GV) migrates towards the protuberance. The GV breaks down after it migrates to the oocyte membrane on the protuberance side. The protuberance then contracts back into the oocyte and the first polar body extrudes from the site of the former protuberance. The second polar body forms beneath the first. Thus the oocyte protuberance indicates the presumptive animal pole well before maturation of the oocyte.展开更多
文摘通过主成因判别分析法(discriminant analysis of principal component,DAPC)建立仿刺参个体水平地理亚群体来源的判别模型。实验运用了20个微卫星引物,对俄罗斯海参崴(RS)和中国大连两个亚群(CN1、CN2)3个仿刺参地理群体进行了产物扩增和标记分型,获得3个地理群体分子标记表型数据。开创性地运用主成因分析和判别分析法建立判别模型,以研究不同群体仿刺参个体水平上遗传背景及其群体来源。结果显示,运用20对微卫星引物在仿刺参不同地理群体中稳定扩增的136个等位基因位点数据建立的判别模型可以准确判别刺参个体的群体来源。用75%的原有数据集建立模型,剩余的25%数据对模型的准确度进行验证,3个群体时模型准确率大于80%,2个群体时模型准确性超过90%。传统的遗传多样性和遗传结构分析显示,刺参的3个地理群体间存在中度的遗传分化,且遗传距离较近(D1=0.18,D2=0.159)。传统分析方法和新方法结果良好的一致性表明,利用DAPC和微卫星等位基因信息建立的刺参地理群体判别模型,可以很好地用于未知遗传背景刺参种质的来源鉴别。
基金Supported by the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A412)the National Natural Science Foundation of China(No.31072230)
文摘Environmental conditions, including ambient temperature, play important roles in survival growth development, and reproduction of the Japanese sea cucumber, Apostichopus japonicus. Low temperatures result in slowed growth and skin ulceration disease. In a previous study, we investigated the effect of low temperature on gene expression profiles inA.japonicus by suppression subtractive hybridization （SSH）. Genes encoding Ferritin, Lysozyme, Hsp70, gp96, and AjToll were selected from a subtracted cDNA library of A. japonicus under acute cold stress. The transcriptional expression profiles of these genes were investigated in different tissues （coelomocyte, respiratory tree, intestine, longitudinal muscle） after exposure to acute and mild temperature dropping treatments. The results show that （1） the five cold-tolerance-related genes were found in all four tissues and the highest mRNA levels were observed in coelomocyte and respiratory tree; （2） under the temperature dropping treatments, three types of transcriptional regulation patterns were observed： primary suppression followed by up-regulation at -2℃, suppressed expression throughout the two treatments, and more rarely an initial stimulation followed by suppression; and （3） gene expression suppression was more severe under acute temperature dropping than under mild temperature dropping treatment. The five cold-tolerance-related genes that were ,distributed mainly in coelomocyte and respiratory tissues were generally down-regulated by low temperature stress but an inverse up-regulation event was found at the extreme temperature （-2℃）.
基金Supported by National High Technology Research and Development Program of China (863 Program) (No. 2006AA10A411)National Programs for Science and Technology Development (No. 2006BAD09A01)
文摘Fully grown oocytes of Apostichopus japonicus have a cytoplasmic protuberance where the oocyte attaches to the follicle. The protuberance and the oolamina located on the opposite side of the oocyte indicate the animal-vegetal axis. Two pre-meiotic centrosomes are anchored to the protuberance by microtubules between centrosomes and protuberance. After meiosis reinitiation induced by DTT solution, the germinal vesicle (GV) migrates towards the protuberance. The GV breaks down after it migrates to the oocyte membrane on the protuberance side. The protuberance then contracts back into the oocyte and the first polar body extrudes from the site of the former protuberance. The second polar body forms beneath the first. Thus the oocyte protuberance indicates the presumptive animal pole well before maturation of the oocyte.
