目的建立葛根芩连片指纹图谱,并采用一测多评法(quantitative analysis of multi-components by single-marker,QAMS)测定其中3’-羟基葛根素、葛根素、3’-甲氧基葛根素、大豆苷、黄芩苷、盐酸巴马汀、盐酸小檗碱、汉黄芩苷、黄芩素、...目的建立葛根芩连片指纹图谱,并采用一测多评法(quantitative analysis of multi-components by single-marker,QAMS)测定其中3’-羟基葛根素、葛根素、3’-甲氧基葛根素、大豆苷、黄芩苷、盐酸巴马汀、盐酸小檗碱、汉黄芩苷、黄芩素、甘草酸铵、汉黄芩素11个化学成分含量。方法HPLC测定10批葛根芩连片的指纹图谱,建立指纹图谱共有模式,以黄芩苷为参照,计算该成分与3’-羟基葛根素、葛根素、3’-甲氧基葛根素、大豆苷、盐酸巴马汀、盐酸小檗碱、汉黄芩苷、黄芩素、甘草酸铵、汉黄芩素的相对校正因子,并计算其含量。同时对QAMS的计算值与外标法实测值进行比较,以确证QAMS的可行性和科学性。指纹图谱及含量测定色谱条件:采用Waters Xbridge-C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸水溶液,梯度洗脱;体积流量1.0 mL·min^(-1);柱温30℃;检测波长260 nm。结果10批葛根芩连片标定了共有峰20个,指认了其中11个化学成分,10批葛根芩连片相似度均>0.97,3’-羟基葛根素、葛根素、3’-甲氧基葛根素、大豆苷、黄芩苷、盐酸巴马汀、盐酸小檗碱、汉黄芩苷、黄芩素、甘草酸铵、汉黄芩素的线性范围分别为0.0566~2.8302,0.2412~12.0586,0.1280~6.4010,0.0597~2.9835,0.2427~12.1349,0.0457~2.2857,0.1928~9.6410,0.0433~2.1670,0.0180~0.9002,0.0210~1.0484,0.0115~0.5754μg,r^(2)为0.9996~1.000,平均回收率分别100.23%,102.01%,101.66%,102.73%,100.17%,98.45%,98.41%,100.95%,101.85%,97.97%和100.09%,RSD分别为1.24%~2.57%(n=6);3’-羟基葛根素、葛根素、3’-甲氧基葛根素、大豆苷、盐酸巴马汀、盐酸小檗碱、汉黄芩苷、黄芩素、甘草酸铵、汉黄芩素的相对校正因子分别为0.8604,0.6053,0.8509,0.5828,0.5571,0.4986,0.7672,0.6521,2.6081,0.5452,待测成分含量QAMS与外标法实测值RAD为0.03%~2.12%。结论QAMS结合指纹图谱可为葛根芩连片的定量测定和质量控制提供参考。展开更多
