A laser scanning confocal imaging-surface plasmon resonance （LSCI-SPR） instrument integrated with a wavelength-dependent surface plasmon resonance （SPR） sensor and a laser scanning confocal microscopy （LSCM） is ...A laser scanning confocal imaging-surface plasmon resonance （LSCI-SPR） instrument integrated with a wavelength-dependent surface plasmon resonance （SPR） sensor and a laser scanning confocal microscopy （LSCM） is built to detect the bonding process of human IgG and fluorescent-labeled affinity purified antibodies in real time. The shifts of resonant wavelength at different reaction time stages are obtained by SPR, corresponding well with the changes of the fluorescence intensity collected by using LSCM. The instrument shows the merits of the combination and complementation of the SPR and LSCM, with such advantages as quantificational analysis, high spatial resolution and real time monitor, which are of great importance for practical applications in biosensor and life science.展开更多
患者 男,36岁,于就诊4个月前因左腕部电锯伤在当地医院行急诊手术,术中发现尺神经断裂并行神经修复术。术后自觉运动感觉无任何进步,至门诊检查发现左手爪形手畸形,背侧骨间肌萎缩明显,但反复行感觉检查后仍自述环、小指掌侧感觉存在。...患者 男,36岁,于就诊4个月前因左腕部电锯伤在当地医院行急诊手术,术中发现尺神经断裂并行神经修复术。术后自觉运动感觉无任何进步,至门诊检查发现左手爪形手畸形,背侧骨间肌萎缩明显,但反复行感觉检查后仍自述环、小指掌侧感觉存在。患者自评DASH(the Disabilities of the Arm.Shoulder,and Hand)评分为9.2分,视觉模拟评分(visual analog scale,VAS)为4分。因就诊医院无肌电图及神经B超,故建议患者至上级医院做进一步尺神经损伤定位及定性检查。展开更多
基金supported by the Instrument Developing Project of the Chinese Academy of Sciences (Grant No.YZ200740)the National Natural Science Foundation of China (Grant Nos.60978034 and 10974019)the National High Technology Research and Development Program of China (Grant No.2009AA03Z318)
文摘A laser scanning confocal imaging-surface plasmon resonance （LSCI-SPR） instrument integrated with a wavelength-dependent surface plasmon resonance （SPR） sensor and a laser scanning confocal microscopy （LSCM） is built to detect the bonding process of human IgG and fluorescent-labeled affinity purified antibodies in real time. The shifts of resonant wavelength at different reaction time stages are obtained by SPR, corresponding well with the changes of the fluorescence intensity collected by using LSCM. The instrument shows the merits of the combination and complementation of the SPR and LSCM, with such advantages as quantificational analysis, high spatial resolution and real time monitor, which are of great importance for practical applications in biosensor and life science.
文摘患者 男,36岁,于就诊4个月前因左腕部电锯伤在当地医院行急诊手术,术中发现尺神经断裂并行神经修复术。术后自觉运动感觉无任何进步,至门诊检查发现左手爪形手畸形,背侧骨间肌萎缩明显,但反复行感觉检查后仍自述环、小指掌侧感觉存在。患者自评DASH(the Disabilities of the Arm.Shoulder,and Hand)评分为9.2分,视觉模拟评分(visual analog scale,VAS)为4分。因就诊医院无肌电图及神经B超,故建议患者至上级医院做进一步尺神经损伤定位及定性检查。
