[ Objective] The aim was to seek a simple and quick method of extracting genomic DNA from wheat leaves. [ Method] Taking tender leaves of wheat as test materials, total DNA of transgenic wheat was extracted by using m...[ Objective] The aim was to seek a simple and quick method of extracting genomic DNA from wheat leaves. [ Method] Taking tender leaves of wheat as test materials, total DNA of transgenic wheat was extracted by using modified CTAB method. The extracted DNA was detected by 0.8% agarose gel electrophoresis. [ Result] DNA purity of extracted genome DNA from wheat was high and no degradation phenomenon using modified CTAB method, and was suitable for carrying out normal PCR amplification. [ Conclusion] This study provides a simple and quick method for extracting DNA from wheat with a spot of material.展开更多
基金Supported by National GMO Cultivation of New Varieties of Major Projects "Anti-reverse to Cultivate New Varieties of Genetically Modified Wheat," a Major IssueNational Science and Technology Support Program Topics (2006BAD01A02-8)National System of Industrial Science and Technology of Modern Wheat Comprehensive Experimental Station in Shanxi Province and National public Service Sector(Agriculture) Research Project (Shanxi Province)(nycytx-03)~~
文摘[ Objective] The aim was to seek a simple and quick method of extracting genomic DNA from wheat leaves. [ Method] Taking tender leaves of wheat as test materials, total DNA of transgenic wheat was extracted by using modified CTAB method. The extracted DNA was detected by 0.8% agarose gel electrophoresis. [ Result] DNA purity of extracted genome DNA from wheat was high and no degradation phenomenon using modified CTAB method, and was suitable for carrying out normal PCR amplification. [ Conclusion] This study provides a simple and quick method for extracting DNA from wheat with a spot of material.
