目的运用全面触发工具(global trigger tool,GTT)初步建立新疆某医院肾移植受者免疫抑制药品不良事件(adverse drug events,ADE)的主动监测模型。方法采用触发器进行回顾性审查病例324份,对病例中ADE及其分类、分级进行判定,并采用Logis...目的运用全面触发工具(global trigger tool,GTT)初步建立新疆某医院肾移植受者免疫抑制药品不良事件(adverse drug events,ADE)的主动监测模型。方法采用触发器进行回顾性审查病例324份,对病例中ADE及其分类、分级进行判定,并采用Logistic分析探讨与ADE发生可能相关的因素,初步建立ADE主动监测模型。结果39项触发器全部触发,总体阳性预测值(PPV%)为27.74%,触发器阳性触发率97.22%。判定出564个ADE,触发器ADE检出率68.52%(222/324),ADE/100例患者为174.07,ADE/1000患者天是32.03。ADE累及系统-器官13类,主要是代谢和营养障碍(30.67%)、血液系统(17.2%)、机会性感染(15.07%)。二分类Logistic分析显示7个因素是肾移植受者发生ADE的独立危险因素。在此基础上建立了免疫抑制剂ADE个体化列线图模型,方法学考察表明该模型具有很好的预测价值(AUC为0.863)。结论全面触发工具可用于肾移植受者免疫抑制ADE的监测,但需进一步完善触发器并进行验证研究。展开更多
Curcumin, as a main pharmacological component in the traditional Chinese medicine-- tttrmeric, has shown anti-inflammatory, anti-oxidation, anti-tumor and anti-fibrotic effects. This study aimed to investigate the pos...Curcumin, as a main pharmacological component in the traditional Chinese medicine-- tttrmeric, has shown anti-inflammatory, anti-oxidation, anti-tumor and anti-fibrotic effects. This study aimed to investigate the possible underlying signaling pathway which was involved in the inhibition of LDL-induced proliferation of mesangial cells and matrix by curcumin. Rat mesangial cells in vitro were incubated with low-density lipoprotein （LDL） and different concentrations of curcumin （0, 6.25, 12.5, 25.0 9mol/L） or p38 MAPK inhibitor, SB203580 （10 μmol/L）. Under LDL incubation, mesangial cells proliferated, the expression of MMP-2 mRNA and protein was decreased, the expression of COX-2 mRNA and protein was increased, reactive oxygen species （ROS） generation was increased and p38 MAPK was activated significantly （P〈0.05）. When LDL-induced cells were treated with curcumin in the concentration of 12.5 or 25.0 μmol/L, LDL-induced proliferation ofmesangial cells was suppressed, the expression of MMP-2 mRNA and protein increased, the expression of COX-2 mRNA and protein downregulated, the production of ROS inhibited and p38 MAPK inactivated （P〈0.05）. In conclusion, curcumin can inhibit the LDL-induced proliferation of mesangial cells and up-regulate the expression of MMP-2, which may be related with the inhibitory effect of curcumin on COX-2 expression, ROS pro- duction and p38 MAPK.展开更多
文摘目的运用全面触发工具(global trigger tool,GTT)初步建立新疆某医院肾移植受者免疫抑制药品不良事件(adverse drug events,ADE)的主动监测模型。方法采用触发器进行回顾性审查病例324份,对病例中ADE及其分类、分级进行判定,并采用Logistic分析探讨与ADE发生可能相关的因素,初步建立ADE主动监测模型。结果39项触发器全部触发,总体阳性预测值(PPV%)为27.74%,触发器阳性触发率97.22%。判定出564个ADE,触发器ADE检出率68.52%(222/324),ADE/100例患者为174.07,ADE/1000患者天是32.03。ADE累及系统-器官13类,主要是代谢和营养障碍(30.67%)、血液系统(17.2%)、机会性感染(15.07%)。二分类Logistic分析显示7个因素是肾移植受者发生ADE的独立危险因素。在此基础上建立了免疫抑制剂ADE个体化列线图模型,方法学考察表明该模型具有很好的预测价值(AUC为0.863)。结论全面触发工具可用于肾移植受者免疫抑制ADE的监测,但需进一步完善触发器并进行验证研究。
基金supported by grants from the National Natural Science Foundation of China(No.81100485)Scientific Research Foundation for Returned Scholars, Ministry of Education of China(No.[2011]1568)
文摘Curcumin, as a main pharmacological component in the traditional Chinese medicine-- tttrmeric, has shown anti-inflammatory, anti-oxidation, anti-tumor and anti-fibrotic effects. This study aimed to investigate the possible underlying signaling pathway which was involved in the inhibition of LDL-induced proliferation of mesangial cells and matrix by curcumin. Rat mesangial cells in vitro were incubated with low-density lipoprotein （LDL） and different concentrations of curcumin （0, 6.25, 12.5, 25.0 9mol/L） or p38 MAPK inhibitor, SB203580 （10 μmol/L）. Under LDL incubation, mesangial cells proliferated, the expression of MMP-2 mRNA and protein was decreased, the expression of COX-2 mRNA and protein was increased, reactive oxygen species （ROS） generation was increased and p38 MAPK was activated significantly （P〈0.05）. When LDL-induced cells were treated with curcumin in the concentration of 12.5 or 25.0 μmol/L, LDL-induced proliferation ofmesangial cells was suppressed, the expression of MMP-2 mRNA and protein increased, the expression of COX-2 mRNA and protein downregulated, the production of ROS inhibited and p38 MAPK inactivated （P〈0.05）. In conclusion, curcumin can inhibit the LDL-induced proliferation of mesangial cells and up-regulate the expression of MMP-2, which may be related with the inhibitory effect of curcumin on COX-2 expression, ROS pro- duction and p38 MAPK.
