According to the published genome sequences of Porcine circovirus type 2 (PCV2) and Porcine reproductive and respiratory syndrome virus (PRRSV), primers were designed and PCR, RT-PCR were set up for the detection of P...According to the published genome sequences of Porcine circovirus type 2 (PCV2) and Porcine reproductive and respiratory syndrome virus (PRRSV), primers were designed and PCR, RT-PCR were set up for the detection of PCV2 and PRRSV, respectively. With the established methods, 38 clinical samples from the respiratory disease pigs were detected for the presence of PCV2 and PRRSV. The results demonstrated that 22 samples were positive for PCV2, 27 samples were positive for PRRSV and among the above positive samples, 18 samples were positive for both viruses. The data obtained in the present study indicated that PCV2 and PRRSV maybe play an important role in the course of the development of respiratory diseases.展开更多
为探索检测管制疲劳指标的性能,利用模拟塔台管制软件和眼动仪搭建实验平台进行实验,设计了扫视速度、注视点数、平均注视时长、扫视幅度、闪光融合频率和眼闭合时间比例(percentage of eyelid closure time,PERCLOS)6项指标;并利用卡...为探索检测管制疲劳指标的性能,利用模拟塔台管制软件和眼动仪搭建实验平台进行实验,设计了扫视速度、注视点数、平均注视时长、扫视幅度、闪光融合频率和眼闭合时间比例(percentage of eyelid closure time,PERCLOS)6项指标;并利用卡罗林斯卡嗜睡量表对主观疲劳程度进行评定。通过疲劳前后各项指标的差异显著性,从与疲劳的相关性、受试者工作特征曲线两个方面进行分析,分别评估了指标单项检测和联合检测管制疲劳的性能。研究结果表明:扫视速度、注视点数、闪光融合频率和PERCLOS指标与疲劳呈中度及以上显著相关;其检测管制疲劳的性能在中等及以上水平,可以作为检测管制疲劳的参考指标。指标联合检测管制疲劳的性能要优于单项检测,可以更为准确地检测管制疲劳。所有指标中PERCLOS指标是检测管制疲劳指标中性能最好的指标。展开更多
文摘According to the published genome sequences of Porcine circovirus type 2 (PCV2) and Porcine reproductive and respiratory syndrome virus (PRRSV), primers were designed and PCR, RT-PCR were set up for the detection of PCV2 and PRRSV, respectively. With the established methods, 38 clinical samples from the respiratory disease pigs were detected for the presence of PCV2 and PRRSV. The results demonstrated that 22 samples were positive for PCV2, 27 samples were positive for PRRSV and among the above positive samples, 18 samples were positive for both viruses. The data obtained in the present study indicated that PCV2 and PRRSV maybe play an important role in the course of the development of respiratory diseases.
文摘为探索检测管制疲劳指标的性能,利用模拟塔台管制软件和眼动仪搭建实验平台进行实验,设计了扫视速度、注视点数、平均注视时长、扫视幅度、闪光融合频率和眼闭合时间比例(percentage of eyelid closure time,PERCLOS)6项指标;并利用卡罗林斯卡嗜睡量表对主观疲劳程度进行评定。通过疲劳前后各项指标的差异显著性,从与疲劳的相关性、受试者工作特征曲线两个方面进行分析,分别评估了指标单项检测和联合检测管制疲劳的性能。研究结果表明:扫视速度、注视点数、闪光融合频率和PERCLOS指标与疲劳呈中度及以上显著相关;其检测管制疲劳的性能在中等及以上水平,可以作为检测管制疲劳的参考指标。指标联合检测管制疲劳的性能要优于单项检测,可以更为准确地检测管制疲劳。所有指标中PERCLOS指标是检测管制疲劳指标中性能最好的指标。
