In order to investigate the possible role of macrophages in pathological changes of cerebrovascular diseases, we observed and counted the numbers of perivascular macrophages in brains of spontaneously hypertensive rat...In order to investigate the possible role of macrophages in pathological changes of cerebrovascular diseases, we observed and counted the numbers of perivascular macrophages in brains of spontaneously hypertensive rats(SHR) and normotensive Wistar-Kyoto(WKY)rats using monoclon al antibodies(ED2)against rat macrophages.The immunohistochemistry(ABC method)and perfu sion-fixed frozen brain sections(16μm)were used in the study.The results showed that the ED2-specific staining in brain sections was restricted to macrophages in a perivascular location.The number of perivascular macrophages was significantly greater in SHR than in WKY.The results suggest that the hypertension is asseciated with increased subendothelial accumulation of macrophages.展开更多
文摘In order to investigate the possible role of macrophages in pathological changes of cerebrovascular diseases, we observed and counted the numbers of perivascular macrophages in brains of spontaneously hypertensive rats(SHR) and normotensive Wistar-Kyoto(WKY)rats using monoclon al antibodies(ED2)against rat macrophages.The immunohistochemistry(ABC method)and perfu sion-fixed frozen brain sections(16μm)were used in the study.The results showed that the ED2-specific staining in brain sections was restricted to macrophages in a perivascular location.The number of perivascular macrophages was significantly greater in SHR than in WKY.The results suggest that the hypertension is asseciated with increased subendothelial accumulation of macrophages.
