Preliminary study has been made to test wether three strains McAb（1B1,5D<sub>6</sub>, 6D<sub>8</sub>）are against the same antigen determinant.Througn ELISA additive and competition expeiments...Preliminary study has been made to test wether three strains McAb（1B1,5D<sub>6</sub>, 6D<sub>8</sub>）are against the same antigen determinant.Througn ELISA additive and competition expeiments,itproved that these three strains are against different antigen determinant.The result of positiveserum antigen component analysis with 2 strains IBDV McAb showed that sample IBD positiveserum had obvious inhibition against combination of 5D<sub>6</sub> McAb with corresponding antigen.Theresults of substitution of corresponding component in ELISA inhibition experiment and compari-son of non-IBD serum （SPF chicken serum,ND,MD,IA positive serum）proved that IBD anti-serum was the only one showing inhibition against 5D<sub>6</sub> McAb.Comparison with AGP and electro-microscope observation showed that ELISA inhibition experiment was characterised by high-specificity,rapidity and sensitiveness.799 serum samples were tested with ELISA inhibition anddouble immunodiffusion（AGP） experiments.ELISA had gotten 486 positive,with positive rate of60.83%;and AGP 334。展开更多
文摘Preliminary study has been made to test wether three strains McAb（1B1,5D<sub>6</sub>, 6D<sub>8</sub>）are against the same antigen determinant.Througn ELISA additive and competition expeiments,itproved that these three strains are against different antigen determinant.The result of positiveserum antigen component analysis with 2 strains IBDV McAb showed that sample IBD positiveserum had obvious inhibition against combination of 5D<sub>6</sub> McAb with corresponding antigen.Theresults of substitution of corresponding component in ELISA inhibition experiment and compari-son of non-IBD serum （SPF chicken serum,ND,MD,IA positive serum）proved that IBD anti-serum was the only one showing inhibition against 5D<sub>6</sub> McAb.Comparison with AGP and electro-microscope observation showed that ELISA inhibition experiment was characterised by high-specificity,rapidity and sensitiveness.799 serum samples were tested with ELISA inhibition anddouble immunodiffusion（AGP） experiments.ELISA had gotten 486 positive,with positive rate of60.83%;and AGP 334。
