A spectrophotometric method for the determination of cysteine was developed basing on the reaction of cysteine with cobalt (Ⅱ) in NH 4Cl NH 4OH. The molar absorptivity is 7.98×10 3 L·mol -1 ·cm...A spectrophotometric method for the determination of cysteine was developed basing on the reaction of cysteine with cobalt (Ⅱ) in NH 4Cl NH 4OH. The molar absorptivity is 7.98×10 3 L·mol -1 ·cm -1 at 284 nm. Beer′s law is obeyed for cysteine in the range of 0～2.0×10 -4 mol·L -1 . The method was applied to the assay of cysteine in urine.展开更多
文摘A spectrophotometric method for the determination of cysteine was developed basing on the reaction of cysteine with cobalt (Ⅱ) in NH 4Cl NH 4OH. The molar absorptivity is 7.98×10 3 L·mol -1 ·cm -1 at 284 nm. Beer′s law is obeyed for cysteine in the range of 0～2.0×10 -4 mol·L -1 . The method was applied to the assay of cysteine in urine.
