A novel pulsed-electrospray device is reported in this paper. With such an apparatus, the observation of the electrospray process under the pulsed high voltage conditions has been successfully made with microscope, os...A novel pulsed-electrospray device is reported in this paper. With such an apparatus, the observation of the electrospray process under the pulsed high voltage conditions has been successfully made with microscope, oscilloscope and mass spectrometer. By using pulsed spray technique, the sample flow rate can be averagely decreased to ＜100 nL/min. After increasing the frequency of pulsed high voltage, it is hopefully to directly combine this device to time-of-flight mass spectrometer and obtain a great enhancement both in signal-to-noise ratio and in detection sensitivity for mass spectrometry analysis.展开更多
The technique of coupling capillary liquid chromatography (CLC) with ESIMS was introduced in this article. The homemade reverse phase CLC was coupled with ESIMS to analyze the proteolysis of the BSA and TCS. Good sepa...The technique of coupling capillary liquid chromatography (CLC) with ESIMS was introduced in this article. The homemade reverse phase CLC was coupled with ESIMS to analyze the proteolysis of the BSA and TCS. Good separation efficiency is obtained in the peptide mapping and the partial sequence of peptides was deduced in the CID spectrum. Studies show that CLC-MS has high efficiency on sample separation, and has the merits of sensitivity, accuracy and speed on sample identification. CLC has the lower sample consuming and will be more widely used in the proteomics.展开更多
文摘A novel pulsed-electrospray device is reported in this paper. With such an apparatus, the observation of the electrospray process under the pulsed high voltage conditions has been successfully made with microscope, oscilloscope and mass spectrometer. By using pulsed spray technique, the sample flow rate can be averagely decreased to ＜100 nL/min. After increasing the frequency of pulsed high voltage, it is hopefully to directly combine this device to time-of-flight mass spectrometer and obtain a great enhancement both in signal-to-noise ratio and in detection sensitivity for mass spectrometry analysis.
文摘The technique of coupling capillary liquid chromatography (CLC) with ESIMS was introduced in this article. The homemade reverse phase CLC was coupled with ESIMS to analyze the proteolysis of the BSA and TCS. Good separation efficiency is obtained in the peptide mapping and the partial sequence of peptides was deduced in the CID spectrum. Studies show that CLC-MS has high efficiency on sample separation, and has the merits of sensitivity, accuracy and speed on sample identification. CLC has the lower sample consuming and will be more widely used in the proteomics.
