In the paper accepted by Progress in Natural Science, it was demonstrated thatsome 2 ’-hydroxyl groups of ribose in the accepting stem of yeast alanyl tRNA wereimportant for the recognition between this tRNA and its ...In the paper accepted by Progress in Natural Science, it was demonstrated thatsome 2 ’-hydroxyl groups of ribose in the accepting stem of yeast alanyl tRNA wereimportant for the recognition between this tRNA and its aminoacyl-tRNA synthetase.In this note we intend to study the function of ribose in anticodon of yeast alanyltRNA. In the previous work, it was found that the anticodon loop of yeastalanyl tRNA is not involved in the interaction between tRNA and展开更多
Studies so far indicate that many processes in the interactions between biomacromole-cules are accompanied by a change in occupied volume. For instance, the association ofprotein subunits into oligomer and the formati...Studies so far indicate that many processes in the interactions between biomacromole-cules are accompanied by a change in occupied volume. For instance, the association ofprotein subunits into oligomer and the formation of protein-nucleic acid complex give anincrease in volume. The increase in volume in these processes mainly results from: (i) theformation of the dead space between the subunits of the oligomeric protein or the com-plexes in the processes mentioned above; (ii) the formation of salt bonds and (iii) theinteractions between hydrophobic groups. It is known from the Lechatelier principle that ifhigh pressure is applied to an equilibrium system mentioned above, the original展开更多
Since the availability of T4 RNA ligase, a great progress has been made in fields of synthesis of polynueleotides. However, all reactions were carried out in solvents. Though reactions in solvents favour the activity ...Since the availability of T4 RNA ligase, a great progress has been made in fields of synthesis of polynueleotides. However, all reactions were carried out in solvents. Though reactions in solvents favour the activity of enzyme, there still exist certain drawbacks, for instance, it takes a long time for separation and the recovery is展开更多
A thirty-nine nucleotide fragment (nucleotides 38—76) of yeast alanyl tRNA was prepared by using calf spleen phosphodiesterase to delete C_(36)m^1I_(37) from the 3′-half molecule of this tRNA under controlled condit...A thirty-nine nucleotide fragment (nucleotides 38—76) of yeast alanyl tRNA was prepared by using calf spleen phosphodiesterase to delete C_(36)m^1I_(37) from the 3′-half molecule of this tRNA under controlled conditions. Analogs of yeast alanyl tRNA with A, G or C instead of m^1I_(37) were synthesized and their biological activities determined. The results indicated that the aminoacylation activity of these analogs was not affected in the rat liver aminoacyl-tRNA synthetase system, compared with natural yeast alanyl tRNA. However, the incorporation activity (i. e. the activity of transferring alanine into proteins) of these analogs was significantly reduced to 20—30% of that of natural yeast alanyl tRNA in the rabbit reticulocyte lysate cell-free protein synthesizing system.展开更多
基金Project supported by the National Natural Science Foundation of China.
文摘In the paper accepted by Progress in Natural Science, it was demonstrated thatsome 2 ’-hydroxyl groups of ribose in the accepting stem of yeast alanyl tRNA wereimportant for the recognition between this tRNA and its aminoacyl-tRNA synthetase.In this note we intend to study the function of ribose in anticodon of yeast alanyltRNA. In the previous work, it was found that the anticodon loop of yeastalanyl tRNA is not involved in the interaction between tRNA and
文摘Studies so far indicate that many processes in the interactions between biomacromole-cules are accompanied by a change in occupied volume. For instance, the association ofprotein subunits into oligomer and the formation of protein-nucleic acid complex give anincrease in volume. The increase in volume in these processes mainly results from: (i) theformation of the dead space between the subunits of the oligomeric protein or the com-plexes in the processes mentioned above; (ii) the formation of salt bonds and (iii) theinteractions between hydrophobic groups. It is known from the Lechatelier principle that ifhigh pressure is applied to an equilibrium system mentioned above, the original
文摘Since the availability of T4 RNA ligase, a great progress has been made in fields of synthesis of polynueleotides. However, all reactions were carried out in solvents. Though reactions in solvents favour the activity of enzyme, there still exist certain drawbacks, for instance, it takes a long time for separation and the recovery is
基金Project supported by the National Natural Science Foundation of China. Part Ⅱ of this series is Ref. [3].
文摘A thirty-nine nucleotide fragment (nucleotides 38—76) of yeast alanyl tRNA was prepared by using calf spleen phosphodiesterase to delete C_(36)m^1I_(37) from the 3′-half molecule of this tRNA under controlled conditions. Analogs of yeast alanyl tRNA with A, G or C instead of m^1I_(37) were synthesized and their biological activities determined. The results indicated that the aminoacylation activity of these analogs was not affected in the rat liver aminoacyl-tRNA synthetase system, compared with natural yeast alanyl tRNA. However, the incorporation activity (i. e. the activity of transferring alanine into proteins) of these analogs was significantly reduced to 20—30% of that of natural yeast alanyl tRNA in the rabbit reticulocyte lysate cell-free protein synthesizing system.
