Gene therapy,as one of the popular therapies nowadays,has been applied with great success in various diseases.Nevertheless,there are also concerns about the safety,therapeutic efficacy,and applicability of gene therap...Gene therapy,as one of the popular therapies nowadays,has been applied with great success in various diseases.Nevertheless,there are also concerns about the safety,therapeutic efficacy,and applicability of gene therapy.Microbubble-assisted ultrasound-guided gene therapy offers the following incomparable advantages over conventional methods.Initially,this strategy enables the therapeutic nucleic acids to effectively cross the barriers like cell membrane,blood-brain barrier,and blood-retinal barrier that exist in the body and reach regions that cannot be accessed by conventional methods,which extends the scope of applications of gene therapy.Next,it is possible to restrict the zone of gene transfection to the lesion area by controlling the irradiation coverage of ultrasound,hence effectively controlling the risk of off-targeting.Additionally,with the support of various imaging techniques like ultrasound imaging and magnetic resonance imaging,the procedure of gene therapy can be under image guidance,which will dramatically improve the safety of gene therapy.This review will highlight on the advances in ultrasound-enhanced gene delivery strategy,attention to the relevant mechanisms of the strategy,the derived technologies and the related cutting-edge applications.Moreover,we will also have a discussion on the limitations and future prospects associated with this gene delivery strategy.展开更多
基金financially supported by the National Natural Science Foundation of China(81930047 to Dai Z,82102062 to Liu R)Sanming Project of Medicine in Shenzhen(SZSM202111011)。
文摘Gene therapy,as one of the popular therapies nowadays,has been applied with great success in various diseases.Nevertheless,there are also concerns about the safety,therapeutic efficacy,and applicability of gene therapy.Microbubble-assisted ultrasound-guided gene therapy offers the following incomparable advantages over conventional methods.Initially,this strategy enables the therapeutic nucleic acids to effectively cross the barriers like cell membrane,blood-brain barrier,and blood-retinal barrier that exist in the body and reach regions that cannot be accessed by conventional methods,which extends the scope of applications of gene therapy.Next,it is possible to restrict the zone of gene transfection to the lesion area by controlling the irradiation coverage of ultrasound,hence effectively controlling the risk of off-targeting.Additionally,with the support of various imaging techniques like ultrasound imaging and magnetic resonance imaging,the procedure of gene therapy can be under image guidance,which will dramatically improve the safety of gene therapy.This review will highlight on the advances in ultrasound-enhanced gene delivery strategy,attention to the relevant mechanisms of the strategy,the derived technologies and the related cutting-edge applications.Moreover,we will also have a discussion on the limitations and future prospects associated with this gene delivery strategy.
