The contents of syringaresnol diglucoside and geniposidic acid in spring leaves and autumn leaves of Eucommia ulmoides Oliv. in 17 provenances were determined by HPLC. The contents of syringaresnol diglucoside in spri...The contents of syringaresnol diglucoside and geniposidic acid in spring leaves and autumn leaves of Eucommia ulmoides Oliv. in 17 provenances were determined by HPLC. The contents of syringaresnol diglucoside in spring leaves and autumn leaves were 0.147%-0.023% and 0.314%-0.011% respectively. The contents of geniposidic acid in spring leaves was not detectable and in autumn leaves 3.98%-0.37%. The contents of syringaresnol diglucoside and geniposidic acid in autumn leaves were much higher than that in spring leaves. The contents of two components in 17 provenances were also distinctive different.展开更多
A high performance liquid chromatography (HPLC) method was established for simultaneous determina-tion of geniposidic acid, chlorogenic acid and geniposide in eucommia. Detection at 240 nm with a reversed-phasecolumn,...A high performance liquid chromatography (HPLC) method was established for simultaneous determina-tion of geniposidic acid, chlorogenic acid and geniposide in eucommia. Detection at 240 nm with a reversed-phasecolumn, CH3OH volume fraction, acidic additive and pH value of mobile phase were studied for their effects on theseparability of the compounds. The most suitable separation was obtained with isocratic gradient elution systemusing CH3OH-H2O-H3 PO4 (12.00: 87.96: 0.04, volume ratio) at a flow-rate of 1.0 mL/min. Under the experi-mental conditions, the capacity factors of three compounds are in 3-13. The sample is separated rightly. Theanalysis time is 30 min and the retention time of genfposidic acid, chlorogenic acid and geniposide are 6. 7 min,10.5 min and 21 min, respectively.展开更多
文摘The contents of syringaresnol diglucoside and geniposidic acid in spring leaves and autumn leaves of Eucommia ulmoides Oliv. in 17 provenances were determined by HPLC. The contents of syringaresnol diglucoside in spring leaves and autumn leaves were 0.147%-0.023% and 0.314%-0.011% respectively. The contents of geniposidic acid in spring leaves was not detectable and in autumn leaves 3.98%-0.37%. The contents of syringaresnol diglucoside and geniposidic acid in autumn leaves were much higher than that in spring leaves. The contents of two components in 17 provenances were also distinctive different.
基金Project (02JZY3029) supported by the Department of Science and Technology of Hunan Province Pro-ject (2002-772) supported by the Development Planning Commission of Hunan Province
文摘A high performance liquid chromatography (HPLC) method was established for simultaneous determina-tion of geniposidic acid, chlorogenic acid and geniposide in eucommia. Detection at 240 nm with a reversed-phasecolumn, CH3OH volume fraction, acidic additive and pH value of mobile phase were studied for their effects on theseparability of the compounds. The most suitable separation was obtained with isocratic gradient elution systemusing CH3OH-H2O-H3 PO4 (12.00: 87.96: 0.04, volume ratio) at a flow-rate of 1.0 mL/min. Under the experi-mental conditions, the capacity factors of three compounds are in 3-13. The sample is separated rightly. Theanalysis time is 30 min and the retention time of genfposidic acid, chlorogenic acid and geniposide are 6. 7 min,10.5 min and 21 min, respectively.
