In the present study, the cleavage activities of both recombinant and bovine enzymes （trypsin and carboxy peptidase B） were determined using proinsulin fusion protein. The proteolysis products were analyzed with HPL...In the present study, the cleavage activities of both recombinant and bovine enzymes （trypsin and carboxy peptidase B） were determined using proinsulin fusion protein. The proteolysis products were analyzed with HPLC. We found that recombinant enzymes had stronger activities and produced fewer by-products. In addition, recombinant enzymes were more efficient in the production of human insulin than bovine enzymes.展开更多
文摘In the present study, the cleavage activities of both recombinant and bovine enzymes （trypsin and carboxy peptidase B） were determined using proinsulin fusion protein. The proteolysis products were analyzed with HPLC. We found that recombinant enzymes had stronger activities and produced fewer by-products. In addition, recombinant enzymes were more efficient in the production of human insulin than bovine enzymes.
