Phosphorothioate oligodeoxynucleotides (S-ODNs), one of DNA analogs, have some biological properties of native DNA such as in hybridization, aqueous solubility, activation of RNase H and cellular uptake via endocytosi...Phosphorothioate oligodeoxynucleotides (S-ODNs), one of DNA analogs, have some biological properties of native DNA such as in hybridization, aqueous solubility, activation of RNase H and cellular uptake via endocytosis. In addition, they are resistant to hydrolysis by exo- and endo-nucleases. They have become noticeable as antisense DNA analogs in recent years.展开更多
In the process of resolution of DL-amino acids by ligand exchange chromatography, the matrix of the packing has great effect on the resolution efficiency. Lefebvre et al.reported that chiral packing (Ⅰ) with hydrophi...In the process of resolution of DL-amino acids by ligand exchange chromatography, the matrix of the packing has great effect on the resolution efficiency. Lefebvre et al.reported that chiral packing (Ⅰ) with hydrophilic polyacrylamide matrix has展开更多
<正> A simple method has been developed to predict the calmodulin-binding domains of peptides and the affinities of the peptides for calmodulin based on a model for the calmodulin-binding domains of peptides pro...<正> A simple method has been developed to predict the calmodulin-binding domains of peptides and the affinities of the peptides for calmodulin based on a model for the calmodulin-binding domains of peptides proposed in a previous paper and the literature data of calmodulin-binding peptides. The effects of hydrophobitities, α-helix-forming tendencies, and basicities of peptides on their affinities for calmodulin were studied. Model peptides were designed to examine the calmodulin-binding domain model and the prediction method. The determined dissociation constants of the complexes formed from the model peptides and calmodulin are in good agreement with the predicted results.展开更多
基金Project supported by the National Natural Science Foundation of China.
文摘Phosphorothioate oligodeoxynucleotides (S-ODNs), one of DNA analogs, have some biological properties of native DNA such as in hybridization, aqueous solubility, activation of RNase H and cellular uptake via endocytosis. In addition, they are resistant to hydrolysis by exo- and endo-nucleases. They have become noticeable as antisense DNA analogs in recent years.
文摘In the process of resolution of DL-amino acids by ligand exchange chromatography, the matrix of the packing has great effect on the resolution efficiency. Lefebvre et al.reported that chiral packing (Ⅰ) with hydrophilic polyacrylamide matrix has
基金Project supported by the National Natural Science Foundation of China
文摘<正> A simple method has been developed to predict the calmodulin-binding domains of peptides and the affinities of the peptides for calmodulin based on a model for the calmodulin-binding domains of peptides proposed in a previous paper and the literature data of calmodulin-binding peptides. The effects of hydrophobitities, α-helix-forming tendencies, and basicities of peptides on their affinities for calmodulin were studied. Model peptides were designed to examine the calmodulin-binding domain model and the prediction method. The determined dissociation constants of the complexes formed from the model peptides and calmodulin are in good agreement with the predicted results.
