地衣芽孢杆菌(B acillus lichen if orm is)NK-X3产生的脂肽类表面活性剂具有高表面活性,其临界胶束浓度(CM C)值为30 m g/L.采用接触角测量和烃粘附率实验研究低于CM C值的脂肽浓度对细胞表面亲疏水性和粘附性的影响.结果表明,脂肽具...地衣芽孢杆菌(B acillus lichen if orm is)NK-X3产生的脂肽类表面活性剂具有高表面活性,其临界胶束浓度(CM C)值为30 m g/L.采用接触角测量和烃粘附率实验研究低于CM C值的脂肽浓度对细胞表面亲疏水性和粘附性的影响.结果表明,脂肽具有改变细菌表面的疏水特性,使亲水性的NK-X3菌疏水性增强,疏水性的K 80-B菌疏水性减弱.脂肽改变细菌细胞表面的亲疏水性,从而改变细菌的粘附性.展开更多
The affinity heptapeptide(HWWWPAS)for insulin,selected from phage display library,was coupled to EAH Sepharose 4B gel and packed to a 1-mL column.The column was used for the affinity purification of insulin from prote...The affinity heptapeptide(HWWWPAS)for insulin,selected from phage display library,was coupled to EAH Sepharose 4B gel and packed to a 1-mL column.The column was used for the affinity purification of insulin from protein mixture and commercial insulin preparation.It was observed that the minor impurity in the commercial insulin was removed by the affinity chromatography.Nearly 40 mg of insulin could be purified with the 1-mL affinity column.The results revealed the high specificity and capacity of the affinity column for insulin purification.Moreover,based on the analysis of the amino acids in the peptide sequence,shorter peptides were designed and synthesized for insulin chromatography.As a result,HWWPS was found to be a good alternative to HWWWPAS,while the other two peptides with three or four amino acids showed weak affinity for insulin.The results indicated that the peptide sequence of HWWWPAS was quite conservative for specific binding of insulin.展开更多
基金Supported by Natural Science Foundation of China(No.20476081)the Programfor Changjiang ScholarsInnovative Research Team in University from the Ministry of Education of China.
文摘The affinity heptapeptide(HWWWPAS)for insulin,selected from phage display library,was coupled to EAH Sepharose 4B gel and packed to a 1-mL column.The column was used for the affinity purification of insulin from protein mixture and commercial insulin preparation.It was observed that the minor impurity in the commercial insulin was removed by the affinity chromatography.Nearly 40 mg of insulin could be purified with the 1-mL affinity column.The results revealed the high specificity and capacity of the affinity column for insulin purification.Moreover,based on the analysis of the amino acids in the peptide sequence,shorter peptides were designed and synthesized for insulin chromatography.As a result,HWWPS was found to be a good alternative to HWWWPAS,while the other two peptides with three or four amino acids showed weak affinity for insulin.The results indicated that the peptide sequence of HWWWPAS was quite conservative for specific binding of insulin.
