Objective To clarify the distribution of hepatitis A virus (HAV) genotype in geographical regions of China Methods Seventeen representative HAV strains were isolated from the stool or serum of hepatitis A patients...Objective To clarify the distribution of hepatitis A virus (HAV) genotype in geographical regions of China Methods Seventeen representative HAV strains were isolated from the stool or serum of hepatitis A patients in different geographical regions Viral RNA was recovered from stool or serum by proteinase K digestion and phenol chloroform extraction, followed by ethanol precipitation prior to reverse transcription and polymerase chain reaction (RT PCR) amplification The nucleotide sequences of VP1/2A junction region were tested by using a direct sequencing technique Results A pairwise comparison of sequences within 168 bases at the VP1/2A junction revealed that all the sequences clustered within genotype Ⅰ About 53% of strains clustered in genotype ⅠB, with less than 6% variability; while the others clustered in genotype ⅠA, with less than 5 3% variability Sequence homology between genotype ⅠA and ⅠB varied from 88 7% to 92 3% Conclusion Epidemic or sporadic HAV strains in China may belong to HAV genotype ⅠA or ⅠB Epidemiologically related strains may be identical or closely related in sequence展开更多
文摘Objective To clarify the distribution of hepatitis A virus (HAV) genotype in geographical regions of China Methods Seventeen representative HAV strains were isolated from the stool or serum of hepatitis A patients in different geographical regions Viral RNA was recovered from stool or serum by proteinase K digestion and phenol chloroform extraction, followed by ethanol precipitation prior to reverse transcription and polymerase chain reaction (RT PCR) amplification The nucleotide sequences of VP1/2A junction region were tested by using a direct sequencing technique Results A pairwise comparison of sequences within 168 bases at the VP1/2A junction revealed that all the sequences clustered within genotype Ⅰ About 53% of strains clustered in genotype ⅠB, with less than 6% variability; while the others clustered in genotype ⅠA, with less than 5 3% variability Sequence homology between genotype ⅠA and ⅠB varied from 88 7% to 92 3% Conclusion Epidemic or sporadic HAV strains in China may belong to HAV genotype ⅠA or ⅠB Epidemiologically related strains may be identical or closely related in sequence
