Objective： Ce/astrus orbicu/atus Thunb. has been used for thousands of years in China as a remedy against cancer and inflammatory diseases. This study aims to investigate whether C. orbiculatus extract （COE） could ...Objective： Ce/astrus orbicu/atus Thunb. has been used for thousands of years in China as a remedy against cancer and inflammatory diseases. This study aims to investigate whether C. orbiculatus extract （COE） could inhibit angiogenesis, which is the pivotal step in tumor growth, invasiveness, and metastasis. Methods： In this study, the extract from the stem of C. orbiculatus was used. Mouse hepatic carcinoma cells （Hepat-6） were treated with COE in different nontoxic concentrations （10, 20, 40, 80, and 160 μg/mL）. The mRNA and protein expression levels of vascular endothelial growth factor （VEGF） were detected by reverse transcription-polymerase chain reaction （RT-PCR） and Western blot, respectively; the active fractions were further tested on C57BL/6 mice and human umbilical vein endothelial cells （HUVEC） for any antiangiogenic effects. Results： COE significantly inhibited proliferation and induced apoptosis in Hepal-6 cells and inhibited VEGF expression at both mRNA and protein levels. Furthermore, this agent inhibited the formation of the capillary-like structure in primary cultured HUVEC in a dose-dependent manner. In vivo, COE significantly reduced the volume and weight of solid tumors with low adverse effects and decreased tumor angiogenesis. Conclusions： In summary, COE could be used to treat hepatic carcinoma. The mechanisms of the antitumor activity of COE may be due to its effects against tumor angiogenesis by targeting the VEGF protein.展开更多
加速康复外科(enhanced recovery after surgery,ERAS)是近几年应用于临床的外科新理念,通过加强术前宣教、缩短禁食水时间、控制疼痛、优化麻醉方式、术中控制体温、补液量和速度等,减轻患者疼痛、生理和心理创伤应激,使患者快速康复...加速康复外科(enhanced recovery after surgery,ERAS)是近几年应用于临床的外科新理念,通过加强术前宣教、缩短禁食水时间、控制疼痛、优化麻醉方式、术中控制体温、补液量和速度等,减轻患者疼痛、生理和心理创伤应激,使患者快速康复。在整个围术期需要医生、护理人员、麻醉医师、患者及家属均参与并统筹安排,并将这种合作贯穿治疗始终。最近的研究表明ERAS理念在减轻脊柱外科围手术期应激反应有重要作用并取得可喜进展。本文简要总结国内外学者关于ERAS理念在脊柱外科手术围手术期中的新进展,加深对ERAS理念的认识和理解,从而为临床脊柱外科围手术期患者带来益处。展开更多
基金Supported by Plans of Colleges and Universities in Jiangsu Province to Postgraduate Research and Innovation(No.CX09B-321Z)State Administration of Traditional Chinese Medicine of People's Republic of China(No.04-05ZP35)
文摘Objective： Ce/astrus orbicu/atus Thunb. has been used for thousands of years in China as a remedy against cancer and inflammatory diseases. This study aims to investigate whether C. orbiculatus extract （COE） could inhibit angiogenesis, which is the pivotal step in tumor growth, invasiveness, and metastasis. Methods： In this study, the extract from the stem of C. orbiculatus was used. Mouse hepatic carcinoma cells （Hepat-6） were treated with COE in different nontoxic concentrations （10, 20, 40, 80, and 160 μg/mL）. The mRNA and protein expression levels of vascular endothelial growth factor （VEGF） were detected by reverse transcription-polymerase chain reaction （RT-PCR） and Western blot, respectively; the active fractions were further tested on C57BL/6 mice and human umbilical vein endothelial cells （HUVEC） for any antiangiogenic effects. Results： COE significantly inhibited proliferation and induced apoptosis in Hepal-6 cells and inhibited VEGF expression at both mRNA and protein levels. Furthermore, this agent inhibited the formation of the capillary-like structure in primary cultured HUVEC in a dose-dependent manner. In vivo, COE significantly reduced the volume and weight of solid tumors with low adverse effects and decreased tumor angiogenesis. Conclusions： In summary, COE could be used to treat hepatic carcinoma. The mechanisms of the antitumor activity of COE may be due to its effects against tumor angiogenesis by targeting the VEGF protein.
文摘加速康复外科(enhanced recovery after surgery,ERAS)是近几年应用于临床的外科新理念,通过加强术前宣教、缩短禁食水时间、控制疼痛、优化麻醉方式、术中控制体温、补液量和速度等,减轻患者疼痛、生理和心理创伤应激,使患者快速康复。在整个围术期需要医生、护理人员、麻醉医师、患者及家属均参与并统筹安排,并将这种合作贯穿治疗始终。最近的研究表明ERAS理念在减轻脊柱外科围手术期应激反应有重要作用并取得可喜进展。本文简要总结国内外学者关于ERAS理念在脊柱外科手术围手术期中的新进展,加深对ERAS理念的认识和理解,从而为临床脊柱外科围手术期患者带来益处。
