To investigate the effects of hypoxia, soman and their combined ones on PC12 cells. Methods: After the PC12 cells were exposed to an atmosphere containing different concentrations of oxygen and cultured in a medium co...To investigate the effects of hypoxia, soman and their combined ones on PC12 cells. Methods: After the PC12 cells were exposed to an atmosphere containing different concentrations of oxygen and cultured in a medium containing different concentrations of soman, the amount of lactic dehydrogenase (LDH) released by the cells and their survival rate were determined to observe the dose-dependent and time-dependent cytotoxic effects. Student’s t test and two-way ANOVA were employed to determine the statistical differences and interaction between hypoxia and soman exposure. Results: 1) Both hypoxia and soman exposures exerted dose-dependent cytotoxic effects on PC12 cells and the interaction between the two injurious factors was significant; 2)The combined effects of the two factors were equal to the sum of those exerted by each one separately; and the combined application of the two factors resulted in a more severe cytotoxicity than that caused by either agent used singly; 3) The amount of LDH released from PC12 cells could serve as a more sensitive indicator of cytotoxicity than the survival rate of the cells. Conclusion: This study demonstrates the cytotoxic effects of the combined exposure to hypoxia and soman acted in a summative manner, which suggests that the two factors might induce intracellular release of LDH in PC12 cells through different mechanisms.展开更多
文摘To investigate the effects of hypoxia, soman and their combined ones on PC12 cells. Methods: After the PC12 cells were exposed to an atmosphere containing different concentrations of oxygen and cultured in a medium containing different concentrations of soman, the amount of lactic dehydrogenase (LDH) released by the cells and their survival rate were determined to observe the dose-dependent and time-dependent cytotoxic effects. Student’s t test and two-way ANOVA were employed to determine the statistical differences and interaction between hypoxia and soman exposure. Results: 1) Both hypoxia and soman exposures exerted dose-dependent cytotoxic effects on PC12 cells and the interaction between the two injurious factors was significant; 2)The combined effects of the two factors were equal to the sum of those exerted by each one separately; and the combined application of the two factors resulted in a more severe cytotoxicity than that caused by either agent used singly; 3) The amount of LDH released from PC12 cells could serve as a more sensitive indicator of cytotoxicity than the survival rate of the cells. Conclusion: This study demonstrates the cytotoxic effects of the combined exposure to hypoxia and soman acted in a summative manner, which suggests that the two factors might induce intracellular release of LDH in PC12 cells through different mechanisms.
