目的探讨细胞外基质蛋白-1(extracellular matrix protein 1,ECM1)在肝细胞癌(hepatocellular carcinoma,HCC)患者术前血清中的表达水平与肝癌临床病理特征及生存预后间的关系。方法应用酶联免疫吸附法测定HCC患者及健康体检者血...目的探讨细胞外基质蛋白-1(extracellular matrix protein 1,ECM1)在肝细胞癌(hepatocellular carcinoma,HCC)患者术前血清中的表达水平与肝癌临床病理特征及生存预后间的关系。方法应用酶联免疫吸附法测定HCC患者及健康体检者血清中ECM1表达水平,分析血清ECM1表达水平与临床病理特征及生存预后间的关系。结果117例HCC患者及53例正常对照血清ECM1中位表达浓度分别为166.39、108.06pg/ml,差异有统计学意义(Z=-7.805,P〈0.001)。血清ECM1浓度与肿瘤大小(Z=-3.454,P=0.001)、肿瘤结节数(Z=-2.201,P=0.028)、血管侵犯(Z=-4.685,P〈0.001)和TNM分级(Z=-4.610,P〈0.001)有关。ECM1低表达组患者(180pg/ml)的总生存率和无瘤生存率均明显高于高表达组(〉180pg/ml)。应用Cox回归模型进行单因素和多因素分析均表明血清中ECM1水平是影响肝癌患者生存预后的一个独立危险因素。结论血清ECM1表达水平与肝细胞癌患者术后复发和生存预后密切相关,有可能成为肝癌术后复发的预测指标。展开更多
Endomorphin 1(EM 1) and its six analogs which were designed by rationally replacing the 2 /3 amino acid(Aa) of EM 1 were synthesized by using liquid phase peptides synthesis method to study their action of opiate rece...Endomorphin 1(EM 1) and its six analogs which were designed by rationally replacing the 2 /3 amino acid(Aa) of EM 1 were synthesized by using liquid phase peptides synthesis method to study their action of opiate receptor binding(AORB). The order of their affinity intensity for μ opiate receptor(MOR) was EM 1 >[ D Ala 2]EM 1 >[ D Pro 2]EM 1 > EM 1 >[ L Tyr 3]EM >[ L Pro 3]EM >EM The sequence of their selectivity for MOR is EM 1 >[ D Pro 2]EM 1 =[ L Tyr 3]EM >[ D Ala 2]EM 1 =[ L Pro 3]EM >EM The results showed that, comparatively speaking, the 2 Aa was more closely related to the selectivity of EM 1 while the 3 Aa to their affinity, though the different replacement changed the AORB of EM 1 dissimilarly.展开更多
Eg5又名Kif11(kinesin family member 11)是驱动蛋白家族成员之一,在增殖组织细胞中高表达,定位于有丝分裂期的微管,主要与细胞分裂中染色体的定位、中心体的分离以及双极纺锤体的形成和分离有关。Eg5是近年来研究较多的一种驱动蛋白分...Eg5又名Kif11(kinesin family member 11)是驱动蛋白家族成员之一,在增殖组织细胞中高表达,定位于有丝分裂期的微管,主要与细胞分裂中染色体的定位、中心体的分离以及双极纺锤体的形成和分离有关。Eg5是近年来研究较多的一种驱动蛋白分子,已开发出抑制其活性的肿瘤靶向治疗药物。该文介绍Eg5生物学特性、其抑制物在肿瘤靶向治疗中作用及与疾病关系等方面的最新研究进展。展开更多
文摘目的探讨细胞外基质蛋白-1(extracellular matrix protein 1,ECM1)在肝细胞癌(hepatocellular carcinoma,HCC)患者术前血清中的表达水平与肝癌临床病理特征及生存预后间的关系。方法应用酶联免疫吸附法测定HCC患者及健康体检者血清中ECM1表达水平,分析血清ECM1表达水平与临床病理特征及生存预后间的关系。结果117例HCC患者及53例正常对照血清ECM1中位表达浓度分别为166.39、108.06pg/ml,差异有统计学意义(Z=-7.805,P〈0.001)。血清ECM1浓度与肿瘤大小(Z=-3.454,P=0.001)、肿瘤结节数(Z=-2.201,P=0.028)、血管侵犯(Z=-4.685,P〈0.001)和TNM分级(Z=-4.610,P〈0.001)有关。ECM1低表达组患者(180pg/ml)的总生存率和无瘤生存率均明显高于高表达组(〉180pg/ml)。应用Cox回归模型进行单因素和多因素分析均表明血清中ECM1水平是影响肝癌患者生存预后的一个独立危险因素。结论血清ECM1表达水平与肝细胞癌患者术后复发和生存预后密切相关,有可能成为肝癌术后复发的预测指标。
文摘Endomorphin 1(EM 1) and its six analogs which were designed by rationally replacing the 2 /3 amino acid(Aa) of EM 1 were synthesized by using liquid phase peptides synthesis method to study their action of opiate receptor binding(AORB). The order of their affinity intensity for μ opiate receptor(MOR) was EM 1 >[ D Ala 2]EM 1 >[ D Pro 2]EM 1 > EM 1 >[ L Tyr 3]EM >[ L Pro 3]EM >EM The sequence of their selectivity for MOR is EM 1 >[ D Pro 2]EM 1 =[ L Tyr 3]EM >[ D Ala 2]EM 1 =[ L Pro 3]EM >EM The results showed that, comparatively speaking, the 2 Aa was more closely related to the selectivity of EM 1 while the 3 Aa to their affinity, though the different replacement changed the AORB of EM 1 dissimilarly.
文摘Eg5又名Kif11(kinesin family member 11)是驱动蛋白家族成员之一,在增殖组织细胞中高表达,定位于有丝分裂期的微管,主要与细胞分裂中染色体的定位、中心体的分离以及双极纺锤体的形成和分离有关。Eg5是近年来研究较多的一种驱动蛋白分子,已开发出抑制其活性的肿瘤靶向治疗药物。该文介绍Eg5生物学特性、其抑制物在肿瘤靶向治疗中作用及与疾病关系等方面的最新研究进展。