A scanning Hartmann test method is proposed and its measurement principle is described. The scanning Hartmann test setup is formed by modifying the Hartmann screen of the conventional Hartmann test setup. With the rot...A scanning Hartmann test method is proposed and its measurement principle is described. The scanning Hartmann test setup is formed by modifying the Hartmann screen of the conventional Hartmann test setup. With the rotation of the scanning Hartmann screen and the improved hole arrangement, the whole information of the lens to be tested in the full aperture can be obtained. The measurement accuracy of the aberration is improved and the local error of the lens can be got. In the method, no change of the Hartmann screen is needed for measuring the lenses of different aperture sizes. Experimental results of aberration measurements of two lenses are given to verify the usefulness of the setup.展开更多
An optical biosensor with up-converting phosphor （UCP） marker is developed for the sensitive rapid immunoassay to the specific biomolecule. UCP can emit visible light when excited by infrared light. Through detectin...An optical biosensor with up-converting phosphor （UCP） marker is developed for the sensitive rapid immunoassay to the specific biomolecule. UCP can emit visible light when excited by infrared light. Through detecting and analyzing the content of UCP particles on the test strip after immunoreaction, the concentration of target analyte in the sample can be obtained. The detection sensitivity to plague IgG is better than 5 ng/ml; to plague FI-Ab is better than 100 pg/ml; to plague Yersinia pestis cell is better than 3 ×10^4 CFU/ml. Good linear response characteristics and an excellent correlation （R^2 ≥ 0.95） have been verified by quantitative detection results. In the practical application, detection results to 167 analytic samples have an excellent consistency with those obtained by reverse hemagglutination test. The up-converting phosphor technology （UPT） based biosensor has stable, reliable, and sensitive performances. It can meet the need of various bioassay applications.展开更多
为使金标免疫分析术能对目标被检物进行高灵敏度快速定量检测,成功研制一种金标免疫试纸条反射式光度计。根据纳米金颗粒对绿光的强烈吸收特性,以绿光激光器为照明光源,以扫描方式测量金标免疫试纸条上经生物反应而结合上去的纳米金颗...为使金标免疫分析术能对目标被检物进行高灵敏度快速定量检测,成功研制一种金标免疫试纸条反射式光度计。根据纳米金颗粒对绿光的强烈吸收特性,以绿光激光器为照明光源,以扫描方式测量金标免疫试纸条上经生物反应而结合上去的纳米金颗粒的分布,通过特定算法计算出样品中目标被检物的浓度。该光度计对人绒毛膜促性腺激素标准样品的检测灵敏度达到5 m IU/m l,在5-500 m IU/m l浓度范围内具有良好的线性响应特性,相关系数R^2-0.99,使用研制的光度计对同一生物反应稳定的金标试纸条进行20次重复测量,测量结果的变异系数（CV）小于2%。该光度计具有快速、稳定、灵敏的工作性能。展开更多
文摘A scanning Hartmann test method is proposed and its measurement principle is described. The scanning Hartmann test setup is formed by modifying the Hartmann screen of the conventional Hartmann test setup. With the rotation of the scanning Hartmann screen and the improved hole arrangement, the whole information of the lens to be tested in the full aperture can be obtained. The measurement accuracy of the aberration is improved and the local error of the lens can be got. In the method, no change of the Hartmann screen is needed for measuring the lenses of different aperture sizes. Experimental results of aberration measurements of two lenses are given to verify the usefulness of the setup.
基金This work was supported by the National Natural Science Foundation of China under Grant No. 60378024.
文摘An optical biosensor with up-converting phosphor （UCP） marker is developed for the sensitive rapid immunoassay to the specific biomolecule. UCP can emit visible light when excited by infrared light. Through detecting and analyzing the content of UCP particles on the test strip after immunoreaction, the concentration of target analyte in the sample can be obtained. The detection sensitivity to plague IgG is better than 5 ng/ml; to plague FI-Ab is better than 100 pg/ml; to plague Yersinia pestis cell is better than 3 ×10^4 CFU/ml. Good linear response characteristics and an excellent correlation （R^2 ≥ 0.95） have been verified by quantitative detection results. In the practical application, detection results to 167 analytic samples have an excellent consistency with those obtained by reverse hemagglutination test. The up-converting phosphor technology （UPT） based biosensor has stable, reliable, and sensitive performances. It can meet the need of various bioassay applications.
文摘为使金标免疫分析术能对目标被检物进行高灵敏度快速定量检测,成功研制一种金标免疫试纸条反射式光度计。根据纳米金颗粒对绿光的强烈吸收特性,以绿光激光器为照明光源,以扫描方式测量金标免疫试纸条上经生物反应而结合上去的纳米金颗粒的分布,通过特定算法计算出样品中目标被检物的浓度。该光度计对人绒毛膜促性腺激素标准样品的检测灵敏度达到5 m IU/m l,在5-500 m IU/m l浓度范围内具有良好的线性响应特性,相关系数R^2-0.99,使用研制的光度计对同一生物反应稳定的金标试纸条进行20次重复测量,测量结果的变异系数（CV）小于2%。该光度计具有快速、稳定、灵敏的工作性能。
