针对风速风向检测仪应具有精度高、可靠性强、检测速度快、检测范围大等特点,设计基于可编程片上系统(System On a Programmable Chip,SOPC)技术的超声波风向风速检测系统.该系统以超声波探测器为风速风向检测传感器,建立超声波风速风...针对风速风向检测仪应具有精度高、可靠性强、检测速度快、检测范围大等特点,设计基于可编程片上系统(System On a Programmable Chip,SOPC)技术的超声波风向风速检测系统.该系统以超声波探测器为风速风向检测传感器,建立超声波风速风向检测算法,实现二维的风速风向检测.该系统对风速风向检测仪的发展具有参考意义.展开更多
Protoplast isolation was relevant for gene manipulation in U lva, and universal protocols have been proposed based on evaluation for various wildly collected species. However, only clonal laboratory cultures were prac...Protoplast isolation was relevant for gene manipulation in U lva, and universal protocols have been proposed based on evaluation for various wildly collected species. However, only clonal laboratory cultures were practical for genetic transformation, and whether applicability of such universal protocol existed for these artificial cultures has never been investigated. In this research, samples in different physiological states or developmental stages were tested in U. prolifera. The results proved that the protoplast yields were strongly dependent on the characteristics of samples. Neither F_v/F_m value nor chlorophyll content exhibited an ideal correlation with the protoplast yields. Alternatively, specific growth rate, coupled with developmental stage, could serve as an ef fective combined index to determine the right time for protoplast isolation. According to this instruction, here we reported the highest yields of protoplast((31.5±1.9)×10~6 cells/g f. wt.) in U. prolifera, following comparison between protocols, and further optimizations on enzyme content, incubation period, starting biomass and pretreatment. This specified protocol for artificially cultured clonal samples could meet the need for protoplast-mediated genetic transformation in U. prolifera.展开更多
文摘针对风速风向检测仪应具有精度高、可靠性强、检测速度快、检测范围大等特点,设计基于可编程片上系统(System On a Programmable Chip,SOPC)技术的超声波风向风速检测系统.该系统以超声波探测器为风速风向检测传感器,建立超声波风速风向检测算法,实现二维的风速风向检测.该系统对风速风向检测仪的发展具有参考意义.
基金Supported by the National Natural Science Foundation of China(No.41776153)the Scientific and Technological Innovation Project financially supported by Qingdao National Laboratory for Marine Science and Technology(No.2016ASKJ02-1)+1 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA11020304)the Postdoctoral Application Research Program funded by Qingdao(No.2016189)
文摘Protoplast isolation was relevant for gene manipulation in U lva, and universal protocols have been proposed based on evaluation for various wildly collected species. However, only clonal laboratory cultures were practical for genetic transformation, and whether applicability of such universal protocol existed for these artificial cultures has never been investigated. In this research, samples in different physiological states or developmental stages were tested in U. prolifera. The results proved that the protoplast yields were strongly dependent on the characteristics of samples. Neither F_v/F_m value nor chlorophyll content exhibited an ideal correlation with the protoplast yields. Alternatively, specific growth rate, coupled with developmental stage, could serve as an ef fective combined index to determine the right time for protoplast isolation. According to this instruction, here we reported the highest yields of protoplast((31.5±1.9)×10~6 cells/g f. wt.) in U. prolifera, following comparison between protocols, and further optimizations on enzyme content, incubation period, starting biomass and pretreatment. This specified protocol for artificially cultured clonal samples could meet the need for protoplast-mediated genetic transformation in U. prolifera.
