Background Chemokine-like factor 1 (CKLF1) was recently identified as a novel cytokine The full-length CKLF1 cDNA contains 530 bp encoding 99 amino acid residues with a CC motif similar to that of other CC family c...Background Chemokine-like factor 1 (CKLF1) was recently identified as a novel cytokine The full-length CKLF1 cDNA contains 530 bp encoding 99 amino acid residues with a CC motif similar to that of other CC family chemokines Recombinant CKLF1 exhibits chemotactic activity on leucocytes and stimulates proliferation of murine skeletal muscle cells We questioned whether CKLF1 could be involved in the pathogenesis of inflammation and proliferation in the lung Therefore we used efficient in vivo gene delivery method to investigate the biological effect of CKLF1 in the murine lung Methods CKLF1-expressing plasmid, pCDI-CKLF1, was constructed and injected into the skeletal muscles followed by electroporation Lung tissues were obtained at the end of week 1,2,3 and 4 respectively after injection The pathological changes in the lungs were observed by light microscope Results A single intramuscular injection of CKLF1 plasmid DNA into BALB/c mice caused dramatic pathological changes in the lungs of treated mice These changes included peribronchial leukocyte infiltration, epithelial shedding, collagen deposition, proliferation of bronchial smooth muscle cells and fibrosis of the lung Conclusions The sustained morphological abnormalities of the bronchial and bronchiolar wall, the acute pneumonitis and interstitial pulmonary fibrosis induced by CKLF1 were similar to phenomena observed in chronic persistent asthma, acute respiratory distress syndrome and severe acute respiratory syndrome These data suggest that CKLF1 may play an important role in the pathogenesis of these important diseases and the study also implies that gene electro-transfer in vivo could serve as a valuable approach for evaluating the function of a novel gene in animals展开更多
目的:探索对Calcofluor White荧光染色法的改良,为活检诊断真菌感染提供一种快速、经济、对比更清晰的染色方法。方法:采用Calcofluor White M2R和苏木素、伊红染液,组合成3种染色步骤,对32例病例进行染色,筛选出适用于活检组织,并且在...目的:探索对Calcofluor White荧光染色法的改良,为活检诊断真菌感染提供一种快速、经济、对比更清晰的染色方法。方法:采用Calcofluor White M2R和苏木素、伊红染液,组合成3种染色步骤,对32例病例进行染色,筛选出适用于活检组织,并且在光镜与荧光镜下能同时观察的改良染色法步骤。结果:光镜下,组织呈现HE染色固有的形态。切换至荧光显微镜下,真菌则被显示为亮蓝色,组织背景呈黄绿色,结果清晰、醒目,对比强烈。结论:改良Calcofluor White荧光染色法能使同一张切片在光镜和荧光显微镜下同时观察,相互对照和定位,是提高真菌检出的好方法,值得在真菌的病理活检工作中广泛使用。展开更多
基金ThisworkwassupportedbythegrantsfromtheGuangdongNaturalScienceFoundationKeyProgram (No 0 20741),theNationalNaturalScienceFoundationofChina (No 3 0 3 70 62 2),theNationalHighTechnologyResearchandDevelopmentProgram (10 2 0 8070499),andtheSARSResearchFoundat
文摘Background Chemokine-like factor 1 (CKLF1) was recently identified as a novel cytokine The full-length CKLF1 cDNA contains 530 bp encoding 99 amino acid residues with a CC motif similar to that of other CC family chemokines Recombinant CKLF1 exhibits chemotactic activity on leucocytes and stimulates proliferation of murine skeletal muscle cells We questioned whether CKLF1 could be involved in the pathogenesis of inflammation and proliferation in the lung Therefore we used efficient in vivo gene delivery method to investigate the biological effect of CKLF1 in the murine lung Methods CKLF1-expressing plasmid, pCDI-CKLF1, was constructed and injected into the skeletal muscles followed by electroporation Lung tissues were obtained at the end of week 1,2,3 and 4 respectively after injection The pathological changes in the lungs were observed by light microscope Results A single intramuscular injection of CKLF1 plasmid DNA into BALB/c mice caused dramatic pathological changes in the lungs of treated mice These changes included peribronchial leukocyte infiltration, epithelial shedding, collagen deposition, proliferation of bronchial smooth muscle cells and fibrosis of the lung Conclusions The sustained morphological abnormalities of the bronchial and bronchiolar wall, the acute pneumonitis and interstitial pulmonary fibrosis induced by CKLF1 were similar to phenomena observed in chronic persistent asthma, acute respiratory distress syndrome and severe acute respiratory syndrome These data suggest that CKLF1 may play an important role in the pathogenesis of these important diseases and the study also implies that gene electro-transfer in vivo could serve as a valuable approach for evaluating the function of a novel gene in animals
文摘目的:探索对Calcofluor White荧光染色法的改良,为活检诊断真菌感染提供一种快速、经济、对比更清晰的染色方法。方法:采用Calcofluor White M2R和苏木素、伊红染液,组合成3种染色步骤,对32例病例进行染色,筛选出适用于活检组织,并且在光镜与荧光镜下能同时观察的改良染色法步骤。结果:光镜下,组织呈现HE染色固有的形态。切换至荧光显微镜下,真菌则被显示为亮蓝色,组织背景呈黄绿色,结果清晰、醒目,对比强烈。结论:改良Calcofluor White荧光染色法能使同一张切片在光镜和荧光显微镜下同时观察,相互对照和定位,是提高真菌检出的好方法,值得在真菌的病理活检工作中广泛使用。
