以结缕草(Z oy sia W illd.)杂交后代为材料,以Z014(马尼拉)、Z129(青岛结缕草)及Z077(“兰引3号”结缕草)为对照,以电解质外渗法和地下茎恢复性生长评价其抗寒性,分析抗寒性、枯萎期及青绿期的回归关系。结果表明:叶片电解质外渗率半...以结缕草(Z oy sia W illd.)杂交后代为材料,以Z014(马尼拉)、Z129(青岛结缕草)及Z077(“兰引3号”结缕草)为对照,以电解质外渗法和地下茎恢复性生长评价其抗寒性,分析抗寒性、枯萎期及青绿期的回归关系。结果表明:叶片电解质外渗率半致死温度(LT50)的变化范围为-5.78^-8.26℃,其中22-2、40-2和40-8的半致死温度均低于对照;叶片半致死温度由低到高,即抗寒性由强到弱依次为22-2>40-2>40-8>Z077>Z129>Z014>40-5>31-3>18-1>18-4>37-1>22-3;地下茎恢复生长试验结果表明,杂交后代在-6℃时全部致死,在-2℃处理后均可以恢复生长,恢复生长的百分率变异较大(14.7%~100%),其中31-3与40-2超过Z014与Z077,22-2超过Z077;供试材料间叶片和地下茎抗寒性的测定结果一致;回归分析结果表明,叶片的半致死温度、青绿期及枯萎期相关不显著。展开更多
Using microsatellite markers with 10 pairs of primers,genetic structure of Oreochromis niloticus,Sarotherodon melanotheron and their hybrids were analyzed.The results suggested that:① Six pairs of primers showed a ri...Using microsatellite markers with 10 pairs of primers,genetic structure of Oreochromis niloticus,Sarotherodon melanotheron and their hybrids were analyzed.The results suggested that:① Six pairs of primers showed a rich polymorphic,which can be used as genetic markers for the genetic diversity study of these tilapias.② The analysis based on the six pairs of primers showed that the average heterozygosity(H)ranged as:O.niloticus ♀× S.melanotheron♂(0.725),S.melanotheron ♀ × O.niloticus ♂(0.692),S.melanotheron(0.537),O.niloticus(0.485).The highest effective numbers of alleles(Ne)existed in O.niloticus♀×S.melanotheron♂(3.79),the lowest existed in S.melanotheron(1.92).The average polymorphism information content(PIC)of 4 tilapias was 0.584,the rank from high to low as,O.niloticus ♀ × S.melanotheron ♂(0.668),S.melanotheron ♀× O.niloticus ♂(0.643),O.niloticus(0.375),S.melanotheron(0.333).③ The fact of enhanced heterozigosity of hybrids suggested that there were the genetic basement of cross breeding in tilapia.展开更多
文摘以结缕草(Z oy sia W illd.)杂交后代为材料,以Z014(马尼拉)、Z129(青岛结缕草)及Z077(“兰引3号”结缕草)为对照,以电解质外渗法和地下茎恢复性生长评价其抗寒性,分析抗寒性、枯萎期及青绿期的回归关系。结果表明:叶片电解质外渗率半致死温度(LT50)的变化范围为-5.78^-8.26℃,其中22-2、40-2和40-8的半致死温度均低于对照;叶片半致死温度由低到高,即抗寒性由强到弱依次为22-2>40-2>40-8>Z077>Z129>Z014>40-5>31-3>18-1>18-4>37-1>22-3;地下茎恢复生长试验结果表明,杂交后代在-6℃时全部致死,在-2℃处理后均可以恢复生长,恢复生长的百分率变异较大(14.7%~100%),其中31-3与40-2超过Z014与Z077,22-2超过Z077;供试材料间叶片和地下茎抗寒性的测定结果一致;回归分析结果表明,叶片的半致死温度、青绿期及枯萎期相关不显著。
文摘Using microsatellite markers with 10 pairs of primers,genetic structure of Oreochromis niloticus,Sarotherodon melanotheron and their hybrids were analyzed.The results suggested that:① Six pairs of primers showed a rich polymorphic,which can be used as genetic markers for the genetic diversity study of these tilapias.② The analysis based on the six pairs of primers showed that the average heterozygosity(H)ranged as:O.niloticus ♀× S.melanotheron♂(0.725),S.melanotheron ♀ × O.niloticus ♂(0.692),S.melanotheron(0.537),O.niloticus(0.485).The highest effective numbers of alleles(Ne)existed in O.niloticus♀×S.melanotheron♂(3.79),the lowest existed in S.melanotheron(1.92).The average polymorphism information content(PIC)of 4 tilapias was 0.584,the rank from high to low as,O.niloticus ♀ × S.melanotheron ♂(0.668),S.melanotheron ♀× O.niloticus ♂(0.643),O.niloticus(0.375),S.melanotheron(0.333).③ The fact of enhanced heterozigosity of hybrids suggested that there were the genetic basement of cross breeding in tilapia.
