以腐烂杨梅为研究对象,采用平板划线分离与斜面纯化技术,筛选出两株编号为YMS-7和YMN-5的细菌,分别提取两株菌的DNA后,扩增16S r DNA序列并克隆测序,获得的基因序列在Ez Bio Cloud数据库中进行同源性比对,构建16S r DNA系统发育树,结合...以腐烂杨梅为研究对象,采用平板划线分离与斜面纯化技术,筛选出两株编号为YMS-7和YMN-5的细菌,分别提取两株菌的DNA后,扩增16S r DNA序列并克隆测序,获得的基因序列在Ez Bio Cloud数据库中进行同源性比对,构建16S r DNA系统发育树,结合生理生化分析,将YMS-7和YMN-5分别鉴定为类芽孢杆菌Paenibacillus agaridevorans与嗜气芽孢杆菌Bacillus aerophilus。展开更多
Epigenetic changes caused by DNA methylation and histone modifications play important roles in the regulation of various cellular processes and development. Recent discoveries of 5-methylcytosine(5m C) oxidation deriv...Epigenetic changes caused by DNA methylation and histone modifications play important roles in the regulation of various cellular processes and development. Recent discoveries of 5-methylcytosine(5m C) oxidation derivatives including 5-hydroxymethylcytosine(5hm C), 5-formylcytsine(5f C) and 5-carboxycytosine(5ca C) in mammalian genome further expand our understanding of the epigenetic regulation. Analysis of DNA modification patterns relies increasingly on sequencing-based profiling methods. A number of different approaches have been established to map the DNA epigenomes with single-base resolution, as represented by the bisulfite-based methods, such as classical bisulfite sequencing(BS-seq), TAB-seq(TET-assisted bisulfite sequencing), ox BS-seq(oxidative bisulfite sequencing) and etc. These methods have been used to generate base-resolution maps of 5m C and its oxidation derivatives in genomic samples. The focus of this review will be to discuss the chemical methodologies that have been developed to detect the cytosine derivatives in the genomic DNA.展开更多
文摘以腐烂杨梅为研究对象,采用平板划线分离与斜面纯化技术,筛选出两株编号为YMS-7和YMN-5的细菌,分别提取两株菌的DNA后,扩增16S r DNA序列并克隆测序,获得的基因序列在Ez Bio Cloud数据库中进行同源性比对,构建16S r DNA系统发育树,结合生理生化分析,将YMS-7和YMN-5分别鉴定为类芽孢杆菌Paenibacillus agaridevorans与嗜气芽孢杆菌Bacillus aerophilus。
基金supported by the National Basic Research Foundation of China(2014CB964900 to Yi Chengqi)the National Natural Science Foundation of China(3127083821472009 to Yi Chengqi)
文摘Epigenetic changes caused by DNA methylation and histone modifications play important roles in the regulation of various cellular processes and development. Recent discoveries of 5-methylcytosine(5m C) oxidation derivatives including 5-hydroxymethylcytosine(5hm C), 5-formylcytsine(5f C) and 5-carboxycytosine(5ca C) in mammalian genome further expand our understanding of the epigenetic regulation. Analysis of DNA modification patterns relies increasingly on sequencing-based profiling methods. A number of different approaches have been established to map the DNA epigenomes with single-base resolution, as represented by the bisulfite-based methods, such as classical bisulfite sequencing(BS-seq), TAB-seq(TET-assisted bisulfite sequencing), ox BS-seq(oxidative bisulfite sequencing) and etc. These methods have been used to generate base-resolution maps of 5m C and its oxidation derivatives in genomic samples. The focus of this review will be to discuss the chemical methodologies that have been developed to detect the cytosine derivatives in the genomic DNA.
