猕猴桃果实软腐病是我国猕猴桃贮藏期的主要病害,在四川、陕西、江西等猕猴桃主产区发生普遍,平均发病率达20%-50%,造成了严重的经济损失。中国、意大利、智利等国家认为葡萄座腔菌Botryosphaeria dothidea是该病的主要病原菌（Zhou et ...猕猴桃果实软腐病是我国猕猴桃贮藏期的主要病害,在四川、陕西、江西等猕猴桃主产区发生普遍,平均发病率达20%-50%,造成了严重的经济损失。中国、意大利、智利等国家认为葡萄座腔菌Botryosphaeria dothidea是该病的主要病原菌（Zhou et al.,2015）,拟茎点霉菌Phomopsis sp.也可造成危害（Luongo et al.,2011）。展开更多
根据供试放线菌‘D74’菌株的菌落形态特征及16S r RNA基因序列分析,确定其为娄彻氏链霉菌(Streptomyces rochei)。采用皿内拮抗试验研究表明,‘D74’对魔芋软腐病菌有较强的拮抗作用,拮抗圈直径为11.0~18.3 mm。用‘D74’无菌发酵滤液1...根据供试放线菌‘D74’菌株的菌落形态特征及16S r RNA基因序列分析,确定其为娄彻氏链霉菌(Streptomyces rochei)。采用皿内拮抗试验研究表明,‘D74’对魔芋软腐病菌有较强的拮抗作用,拮抗圈直径为11.0~18.3 mm。用‘D74’无菌发酵滤液10倍稀释液处理甜瓜种子,其发芽率、简明活力指数、胚轴和胚根长度分别较对照增加34.9%、82.4%、66.4%和31.5%(P【0.05)。盆栽条件下,拌土单接菌剂D74和菌剂与病原菌混接处理对收获期魔芋的相对防效分别为100%和17.0%。菌剂D74在单接和混接条件下能提高盆栽魔芋叶片光合作用、叶片绿色度(SPAD)、生物量及产量,亦能改善魔芋球茎品质。田间小区试验条件下,菌剂D74穴施,魔芋叶片光合指标、生物量及产量较对照增加。研究表明,放线菌‘D74’对魔芋软腐病菌有较强抑制作用,‘D74’活菌制剂对魔芋有良好的防病促生效果,对魔芋品质也有显著的改善作用。展开更多
The soft rot infected by pathogenic bacterium Erwinia aroideae Holland is one of the three serious diseases of Chinese cabbage ( Brassica pekinensis Rupr.). By constructing vector system of high frequency transformati...The soft rot infected by pathogenic bacterium Erwinia aroideae Holland is one of the three serious diseases of Chinese cabbage ( Brassica pekinensis Rupr.). By constructing vector system of high frequency transformation mediated by Agrobacterium tunefaciens EHA105, anti-bacterial peptide gene with strong bactericidal action to pathogenic bacteria was introduced into Chinese cabbage AB-81 self-bred line and the transgenic plants were obtained. PCR and Southern blotting detection showed that target gene was integrated into plant genome of Chinese cabbage. The tests of bacteriostasis action of the extract from transgenic plants in vitro, and the assay of disease-resistant of transgenic plantlets in the tube and the pot by perfusing inoculation with pathogenic bacteria showed obvious resistance to soft rot. This resistance can be a stable heredity by genetic analysis of generations of transgenic plants self-bred, separation ratio of its R, was 3:1. The resistance to Km and disease of soft rot was still kept in the R-5. These results indicated the possibility of breeding new varieties of anti-soft rot Chinese cabbage by transgenic plants as parents.展开更多
文摘猕猴桃果实软腐病是我国猕猴桃贮藏期的主要病害,在四川、陕西、江西等猕猴桃主产区发生普遍,平均发病率达20%-50%,造成了严重的经济损失。中国、意大利、智利等国家认为葡萄座腔菌Botryosphaeria dothidea是该病的主要病原菌（Zhou et al.,2015）,拟茎点霉菌Phomopsis sp.也可造成危害（Luongo et al.,2011）。
文摘根据供试放线菌‘D74’菌株的菌落形态特征及16S r RNA基因序列分析,确定其为娄彻氏链霉菌(Streptomyces rochei)。采用皿内拮抗试验研究表明,‘D74’对魔芋软腐病菌有较强的拮抗作用,拮抗圈直径为11.0~18.3 mm。用‘D74’无菌发酵滤液10倍稀释液处理甜瓜种子,其发芽率、简明活力指数、胚轴和胚根长度分别较对照增加34.9%、82.4%、66.4%和31.5%(P【0.05)。盆栽条件下,拌土单接菌剂D74和菌剂与病原菌混接处理对收获期魔芋的相对防效分别为100%和17.0%。菌剂D74在单接和混接条件下能提高盆栽魔芋叶片光合作用、叶片绿色度(SPAD)、生物量及产量,亦能改善魔芋球茎品质。田间小区试验条件下,菌剂D74穴施,魔芋叶片光合指标、生物量及产量较对照增加。研究表明,放线菌‘D74’对魔芋软腐病菌有较强抑制作用,‘D74’活菌制剂对魔芋有良好的防病促生效果,对魔芋品质也有显著的改善作用。
文摘The soft rot infected by pathogenic bacterium Erwinia aroideae Holland is one of the three serious diseases of Chinese cabbage ( Brassica pekinensis Rupr.). By constructing vector system of high frequency transformation mediated by Agrobacterium tunefaciens EHA105, anti-bacterial peptide gene with strong bactericidal action to pathogenic bacteria was introduced into Chinese cabbage AB-81 self-bred line and the transgenic plants were obtained. PCR and Southern blotting detection showed that target gene was integrated into plant genome of Chinese cabbage. The tests of bacteriostasis action of the extract from transgenic plants in vitro, and the assay of disease-resistant of transgenic plantlets in the tube and the pot by perfusing inoculation with pathogenic bacteria showed obvious resistance to soft rot. This resistance can be a stable heredity by genetic analysis of generations of transgenic plants self-bred, separation ratio of its R, was 3:1. The resistance to Km and disease of soft rot was still kept in the R-5. These results indicated the possibility of breeding new varieties of anti-soft rot Chinese cabbage by transgenic plants as parents.
