The role ofcytochrome c in insect cell apoptosis has drawn considerable atten- tion and has been subject to considerable controversy. In Drosophila, the majority of studies have demonstrated that cytochrome c may not ...The role ofcytochrome c in insect cell apoptosis has drawn considerable atten- tion and has been subject to considerable controversy. In Drosophila, the majority of studies have demonstrated that cytochrome c may not be involved in apoptosis, although there are conflicting reports. Cytochrome c is not released from mitochondria into the cytosol and activation of the initiator caspase Dronc or effector caspase Drice is not associated with cytochrome c during apoptosis in Drosophila SL2 cells or BG2 cells. Cytochrome c failed to induce caspase activation and promote caspase activation in Drosophila cell lysates, but remarkably caused caspase activation in extracts from human cells. Knockdown of cytochrome c does not protect cells from apoptosis and over-expression of cytochrome c also does not promote apoptosis. Structural analysis has revealed that cytochrome c is not required for Dapaf-1 complex assembly. In Lepidoptera, the involvement of cytochrome c in apoptosis has been demonstrated by the accumulating evidence. Cytochrome c re- lease from mitochondria into cytosol has been observed in different cell lines such as Spodoptera frugiperda Sf9, Spodoptera litura S1-1 and Lymantria dispar LdFB. Silencing of cytochrome c expression significantly affected apoptosis and activation of caspase and the addition of cytochrome c to cell-free extracts results in caspase activation, suggesting the activation of caspase is dependent on cytochrome c. Although Apaf-1 has not been identified in Lepidoptera, the inhibitor of apoptosome formation can inhibit apoptosis and caspase activation. Cytochrome c may be exclusively required for Lepidoptera apoptosis.展开更多
近日，中科院武汉病毒所周宁一研究员课题组揭示了邻硝基酚2一单加氧酶（onpA）是一种新的含有细胞色素b5结构域的独特黄素单加氧酶。细胞色素b5结构域对此单加氧酶的功能是必须的。相关结果已发表在《细菌学期刊》（Journal of Bacteri...近日，中科院武汉病毒所周宁一研究员课题组揭示了邻硝基酚2一单加氧酶（onpA）是一种新的含有细胞色素b5结构域的独特黄素单加氧酶。细胞色素b5结构域对此单加氧酶的功能是必须的。相关结果已发表在《细菌学期刊》（Journal of Bacteriology）上。展开更多
文摘The role ofcytochrome c in insect cell apoptosis has drawn considerable atten- tion and has been subject to considerable controversy. In Drosophila, the majority of studies have demonstrated that cytochrome c may not be involved in apoptosis, although there are conflicting reports. Cytochrome c is not released from mitochondria into the cytosol and activation of the initiator caspase Dronc or effector caspase Drice is not associated with cytochrome c during apoptosis in Drosophila SL2 cells or BG2 cells. Cytochrome c failed to induce caspase activation and promote caspase activation in Drosophila cell lysates, but remarkably caused caspase activation in extracts from human cells. Knockdown of cytochrome c does not protect cells from apoptosis and over-expression of cytochrome c also does not promote apoptosis. Structural analysis has revealed that cytochrome c is not required for Dapaf-1 complex assembly. In Lepidoptera, the involvement of cytochrome c in apoptosis has been demonstrated by the accumulating evidence. Cytochrome c re- lease from mitochondria into cytosol has been observed in different cell lines such as Spodoptera frugiperda Sf9, Spodoptera litura S1-1 and Lymantria dispar LdFB. Silencing of cytochrome c expression significantly affected apoptosis and activation of caspase and the addition of cytochrome c to cell-free extracts results in caspase activation, suggesting the activation of caspase is dependent on cytochrome c. Although Apaf-1 has not been identified in Lepidoptera, the inhibitor of apoptosome formation can inhibit apoptosis and caspase activation. Cytochrome c may be exclusively required for Lepidoptera apoptosis.
