Achyranthes bidentata polysaccharides (ABPS), water_soluble polysaccharides, isolated from the roots of Achyranthes bidentata Bl. of Amaranthaceae family, was divided into four parts, named as Con.1, Con.2, Con....Achyranthes bidentata polysaccharides (ABPS), water_soluble polysaccharides, isolated from the roots of Achyranthes bidentata Bl. of Amaranthaceae family, was divided into four parts, named as Con.1, Con.2, Con.3 and Con.4, respectively, by chromatography on DEAE_Sepharose fast_flow column and Sephadex G_100 column in order. Con.1 was the constituent of high molecular weight and the other three were all of low molecular weight. Micro_Kjeldahl analysis showed that Con.1 contained 3.95% of nitrogen and neither did the other three parts. The antisenile effects of the four parts of ABPS were studied with Drosophila melanogaster. Results showed that Con.1 has no antisenile effect and all the others could significantly increase the average body weight by 3.85%-5.47% and significantly prolonged the average lifespan by 2.61%- 3.16% of D. melanogaster at the concentration of 2 or 5 mg/g (ABPS/medium).展开更多
文摘Achyranthes bidentata polysaccharides (ABPS), water_soluble polysaccharides, isolated from the roots of Achyranthes bidentata Bl. of Amaranthaceae family, was divided into four parts, named as Con.1, Con.2, Con.3 and Con.4, respectively, by chromatography on DEAE_Sepharose fast_flow column and Sephadex G_100 column in order. Con.1 was the constituent of high molecular weight and the other three were all of low molecular weight. Micro_Kjeldahl analysis showed that Con.1 contained 3.95% of nitrogen and neither did the other three parts. The antisenile effects of the four parts of ABPS were studied with Drosophila melanogaster. Results showed that Con.1 has no antisenile effect and all the others could significantly increase the average body weight by 3.85%-5.47% and significantly prolonged the average lifespan by 2.61%- 3.16% of D. melanogaster at the concentration of 2 or 5 mg/g (ABPS/medium).
文摘探讨了春夏两季模拟酸雨对杜仲 (Eucommia ulmoides Oliv.)叶氮代谢的几个关键酶及相关物质含量的影响。结果表明 :春夏两季杜仲叶硝酸还原酶 (NR)、谷酰胺合成酶 (GS)、谷氨酸脱氢酶 (GDH)和谷丙转氨酶(GPT)活性在一定 p H值酸雨胁迫下随酸雨 p H值的降低而降低 ,夏季各酶活性下降率比春季高。杜仲叶可溶性蛋白质和总氮含量春夏两季也随酸雨 p H值降低而降低 (夏季可溶性蛋白含量与 p H值呈正相关 ) ,总游离氨基酸含量则随 p H值的降低而升高 (二者呈负相关 )。由此可见 ,酸雨对杜仲叶氮代谢产生了显著影响。
