Objective：To establish a retinal vein occlusion（RVO） animal model and observe the therapeutic effect of a Chinese herbal composition（Fufang Xueshuantong Capsule,复方血栓通胶囊,FXC） in ischemic retinal disease.Met...Objective：To establish a retinal vein occlusion（RVO） animal model and observe the therapeutic effect of a Chinese herbal composition（Fufang Xueshuantong Capsule,复方血栓通胶囊,FXC） in ischemic retinal disease.Methods：Fifteen adult male Sprague-Dawley rats underwent laser photothrombosis to induce RVO on their right eyes and were subsequently randomized to receive FXC（the intervention group,n=7） or placebo treatment（the control group,n=8）.Fundus fluorescein angiography was performed after 2,4 and 8 weeks of treatment.Real-time reverse transcription-PCR was used to quantify the mRNA expression of vascular endothelial growth factor（VEGF） and stromal cell-derived factor-1（SDF-1）.The main outcomes were the mRNA copies of VEGF and SDF-1 and the counts of RVO signs.Results：Laser photothrombosis procedure induced typical lesions of RVO,including hemorrhage,leakage,retinal detachment,capillary non-perfusion,filling defect of retinal vessels,and lateral circulation/dilation of small vessels.The retinal lesions were associated with an increased expression of VEGF（P0.05）.No significant change of SDF-1 expression was noticed.Compared with the control group,the intervention group had numerically fewer RVO lesions at week 2（1.71±0.76 vs.3.50±1.51,t=-2.82,P0.05）.The benefit of intervention remained at weeks 4 and 8.Conclusions：A rat model of laser photothrombosis-induced RVO was established and an increase in the VEGF expression was observed in the retinal lesion.The FXC had therapeutic benefit in improving retinal lesions in the rat model of RVO.展开更多
目的:探讨病理性近视中心凹下脉络膜厚度与后巩膜葡萄肿间的相关性。方法:回顾性连续收集病理性近视患者129例,共258眼。根据B超及眼底检查,将患者分为后巩膜葡萄肿组(52例,80眼)和无后巩膜葡萄肿组(77例,154眼)。采用光学相干断层扫描...目的:探讨病理性近视中心凹下脉络膜厚度与后巩膜葡萄肿间的相关性。方法:回顾性连续收集病理性近视患者129例,共258眼。根据B超及眼底检查,将患者分为后巩膜葡萄肿组(52例,80眼)和无后巩膜葡萄肿组(77例,154眼)。采用光学相干断层扫描仪增强深度扫描模式(optical coherence tomography with enhanced depth imaging,EDI-OCT)测量中心凹下脉络膜厚度(CSFCT)。分析各组研究对象的眼部生物学特征与后巩膜葡萄肿之间的关系。结果:与无后巩膜葡萄肿组患者相比,后巩膜葡萄肿组患者年龄较大、眼轴较长、近视较深及CSFCT较薄(P<0.05)。年龄、性别1∶1配对的病例对照亚组分析提示,后巩膜葡萄肿组患眼眼轴长、CSFCT薄(P<0.001)。多因素logistics回归分析发现,矫正年龄、眼轴和CSFCT后,年龄增加[Δ=5岁,OR(95%CI)=1.319(1.120~1.554);P=0.001]、眼轴变长[Δ=1mm,OR(95%CI)=1.898(1.418~2.541);P<0.001]是后巩膜葡萄肿发生的危险因素;而CSFCT增加[Δ=50μm,OR(95%CI)=0.314(0.174~0.567);P<0.001]是后巩膜葡萄肿发生的保护因素。其中,CSFCT下降诊断后巩膜葡萄肿的曲线下面积(area under the curve,AUC)最高,为0.866(95%CI0.821~0.911,P<0.001);Youden指数最大化时,其诊断后巩膜葡萄肿的灵敏度为90.0%、特异度为68.8%,阳性似然比为2.718。结论:病理性近视伴发后巩膜葡萄肿患者具有眼轴长、脉络膜薄等特点;CSFCT下降诊断后巩膜葡萄肿的价值较高,但不能作为诊断后巩膜葡萄肿的特异性指标。展开更多
基金Supported in part by Science and Technology Commission of Shanghai Municipality(Grant No.074119510)
文摘Objective：To establish a retinal vein occlusion（RVO） animal model and observe the therapeutic effect of a Chinese herbal composition（Fufang Xueshuantong Capsule,复方血栓通胶囊,FXC） in ischemic retinal disease.Methods：Fifteen adult male Sprague-Dawley rats underwent laser photothrombosis to induce RVO on their right eyes and were subsequently randomized to receive FXC（the intervention group,n=7） or placebo treatment（the control group,n=8）.Fundus fluorescein angiography was performed after 2,4 and 8 weeks of treatment.Real-time reverse transcription-PCR was used to quantify the mRNA expression of vascular endothelial growth factor（VEGF） and stromal cell-derived factor-1（SDF-1）.The main outcomes were the mRNA copies of VEGF and SDF-1 and the counts of RVO signs.Results：Laser photothrombosis procedure induced typical lesions of RVO,including hemorrhage,leakage,retinal detachment,capillary non-perfusion,filling defect of retinal vessels,and lateral circulation/dilation of small vessels.The retinal lesions were associated with an increased expression of VEGF（P0.05）.No significant change of SDF-1 expression was noticed.Compared with the control group,the intervention group had numerically fewer RVO lesions at week 2（1.71±0.76 vs.3.50±1.51,t=-2.82,P0.05）.The benefit of intervention remained at weeks 4 and 8.Conclusions：A rat model of laser photothrombosis-induced RVO was established and an increase in the VEGF expression was observed in the retinal lesion.The FXC had therapeutic benefit in improving retinal lesions in the rat model of RVO.
文摘目的:探讨病理性近视中心凹下脉络膜厚度与后巩膜葡萄肿间的相关性。方法:回顾性连续收集病理性近视患者129例,共258眼。根据B超及眼底检查,将患者分为后巩膜葡萄肿组(52例,80眼)和无后巩膜葡萄肿组(77例,154眼)。采用光学相干断层扫描仪增强深度扫描模式(optical coherence tomography with enhanced depth imaging,EDI-OCT)测量中心凹下脉络膜厚度(CSFCT)。分析各组研究对象的眼部生物学特征与后巩膜葡萄肿之间的关系。结果:与无后巩膜葡萄肿组患者相比,后巩膜葡萄肿组患者年龄较大、眼轴较长、近视较深及CSFCT较薄(P<0.05)。年龄、性别1∶1配对的病例对照亚组分析提示,后巩膜葡萄肿组患眼眼轴长、CSFCT薄(P<0.001)。多因素logistics回归分析发现,矫正年龄、眼轴和CSFCT后,年龄增加[Δ=5岁,OR(95%CI)=1.319(1.120~1.554);P=0.001]、眼轴变长[Δ=1mm,OR(95%CI)=1.898(1.418~2.541);P<0.001]是后巩膜葡萄肿发生的危险因素;而CSFCT增加[Δ=50μm,OR(95%CI)=0.314(0.174~0.567);P<0.001]是后巩膜葡萄肿发生的保护因素。其中,CSFCT下降诊断后巩膜葡萄肿的曲线下面积(area under the curve,AUC)最高,为0.866(95%CI0.821~0.911,P<0.001);Youden指数最大化时,其诊断后巩膜葡萄肿的灵敏度为90.0%、特异度为68.8%,阳性似然比为2.718。结论:病理性近视伴发后巩膜葡萄肿患者具有眼轴长、脉络膜薄等特点;CSFCT下降诊断后巩膜葡萄肿的价值较高,但不能作为诊断后巩膜葡萄肿的特异性指标。
