FLOWERING LOCUS T(FT)亚家族在植物生长发育过程中发挥着重要作用。为探究毛竹FT基因的表达特性及生物学功能,本研究通过反转录PCR(RT-PCR)从毛竹中克隆到1个FT同源基因PheFT12a,该基因编码区序列长度为522 bp,包含典型的4个外显子和3...FLOWERING LOCUS T(FT)亚家族在植物生长发育过程中发挥着重要作用。为探究毛竹FT基因的表达特性及生物学功能,本研究通过反转录PCR(RT-PCR)从毛竹中克隆到1个FT同源基因PheFT12a,该基因编码区序列长度为522 bp,包含典型的4个外显子和3个内含子,编码173个氨基酸,编码蛋白包含完整的磷脂酰乙醇胺结合蛋白(PEBP)结构域。系统进化树分析结果显示,PheFT12a与水稻(Oryza sativa)OsFTL12的亲缘关系最近,与OsFTL2(Hd3a)和拟南芥(Arabidopsis thaliana)FT的亲缘关系相对较远。实时荧光定量PCR(qRT-PCR)结果显示,PheFT12a基因在毛竹实生苗叶片中表达最高,茎次之。亚细胞定位结果显示,PheFT12a蛋白定位于细胞核和细胞质,主要富集于细胞核。转化拟南芥ft突变体表型结果显示,PheFT12a能明显回补ft突变体的晚花表型,可提高回补植株的主茎数和侧枝数。本研究为进一步分析PheFT12a的生物学功能提供了参考依据,并为毛竹开花及芽发育的分子机制提供了基础资料。展开更多
Low-temperature sintering(LTS)experiments of UO2 pellets and their results were reported.Moreover,a routine process of LTS for UO2 pellets was primarily established.Being sintered at 1 400 ℃ for 3 h in a partially-ox...Low-temperature sintering(LTS)experiments of UO2 pellets and their results were reported.Moreover,a routine process of LTS for UO2 pellets was primarily established.Being sintered at 1 400 ℃ for 3 h in a partially-oxidative atmosphere,the relative density of the pellet can be up to around 94%.Pellets with such a high density are of benefit for following-up reduction-sintering processes.Orthogonal test indicates that the importance of factors affecting the density decreases in the sequence of partial-oxidative sintering temperature and time,reduction-sintering time and temperature,and sintering atmosphere.It is found that it is helpful to introducing a small amount of water vapor into the sintering atmosphere during the latter stage.It is believed that it is the key factor to raise the O/U ratio of original powder in order to improve the properties of the low-temperature sintered pellets.展开更多
文摘Low-temperature sintering(LTS)experiments of UO2 pellets and their results were reported.Moreover,a routine process of LTS for UO2 pellets was primarily established.Being sintered at 1 400 ℃ for 3 h in a partially-oxidative atmosphere,the relative density of the pellet can be up to around 94%.Pellets with such a high density are of benefit for following-up reduction-sintering processes.Orthogonal test indicates that the importance of factors affecting the density decreases in the sequence of partial-oxidative sintering temperature and time,reduction-sintering time and temperature,and sintering atmosphere.It is found that it is helpful to introducing a small amount of water vapor into the sintering atmosphere during the latter stage.It is believed that it is the key factor to raise the O/U ratio of original powder in order to improve the properties of the low-temperature sintered pellets.
