[Objective] The aim was to explore the genetic differences among F2 progenies of sugarcane.[Method] 11 primers were used for ISSR analysis on 20 F2 hybrids of Erianthus fulvus.[Result] A total of 121 bands were amplif...[Objective] The aim was to explore the genetic differences among F2 progenies of sugarcane.[Method] 11 primers were used for ISSR analysis on 20 F2 hybrids of Erianthus fulvus.[Result] A total of 121 bands were amplified in the PCR reactions,among which 110 were polymorphic bands and the polymorphic rate was 90.91%,indicating that there were abundance genetic variation among F2 progenies derived from cross combinations between cultivated sugarcane and E.fulvus.The average genetic coefficients between Yacheng 89/9 and F1 progenies,E.fulvus and F1 progenies,were 0.71 and 0.54,respectively.The clustering result of F2 progenies showed that all the progenies clustered with female parent and suggested that the genetic material of female parent was predominated in F2 progenies.[Conclusion] The study could provide basis for the application of sugarcane innovation germplasm materials in the breeding of sugarcane.展开更多
基金Supported by National Natural Science Foundation Project of China(30960045)Key and New Products Launch Program of Yunnan Province(2010BB011)+1 种基金Talent Training Program of Yunnan Province(2006PY01-38)Sugarcane Industry Technical System Construction Project of Yunnan Province(2009-2013)~~
文摘[Objective] The aim was to explore the genetic differences among F2 progenies of sugarcane.[Method] 11 primers were used for ISSR analysis on 20 F2 hybrids of Erianthus fulvus.[Result] A total of 121 bands were amplified in the PCR reactions,among which 110 were polymorphic bands and the polymorphic rate was 90.91%,indicating that there were abundance genetic variation among F2 progenies derived from cross combinations between cultivated sugarcane and E.fulvus.The average genetic coefficients between Yacheng 89/9 and F1 progenies,E.fulvus and F1 progenies,were 0.71 and 0.54,respectively.The clustering result of F2 progenies showed that all the progenies clustered with female parent and suggested that the genetic material of female parent was predominated in F2 progenies.[Conclusion] The study could provide basis for the application of sugarcane innovation germplasm materials in the breeding of sugarcane.
