AIM: To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen. METHODS: Hepatitis B viral DNA was isolated from HBV carr...AIM: To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen. METHODS: Hepatitis B viral DNA was isolated from HBV carriers' semen and sera using phenol extraction method and QIAamp DNA blood mini kit (Qiagen, Germany). HBV DNA was detected by conventional PCR and quantified by TaqMan technology-based real-time PCR (quantitative polymerase chain reaction (qPCR)). The detection threshold was 200 copies of HBV DNA for conventional PCR and 10 copies of HBV DNA for real time PCR per reaction. RESULTS: Both methods of phenol extraction and QIAamp DNA blood mini kit were suitable for isolating HBV DNA from semen. The value of the detection thresholds was 500 copies of HBV DNA per mL in the semen. The viral loads were 7.5×10^7 and 1.67×10^7 copies of HBV DNA per mL in two HBV infected patients' sera, while 2.14×10^5 and 3.02×10^5 copies of HBV DNA per mL in the semen. CONCLUSION: Real-time PCR is a more sensitive and accurate method to detect and quantify HBV DNA in the semen.展开更多
本文报告用显微摄像监视系统测定107例(生育组30例、不育组77例)精液的精子泳运速度,生育组25.09±4.36μm/s;不育组18.02±4.62μm/s,两组 t 检验具显著差异(t=6.71,P<0.01)。用相差显微镜测定精子尾部摆动(鞭打)频率,生育...本文报告用显微摄像监视系统测定107例(生育组30例、不育组77例)精液的精子泳运速度,生育组25.09±4.36μm/s;不育组18.02±4.62μm/s,两组 t 检验具显著差异(t=6.71,P<0.01)。用相差显微镜测定精子尾部摆动(鞭打)频率,生育组7.85±1.29Hz;不育组6.09±11.36Hz,两组 t 检验具显著差异(t=5.88,P<0.01)。精子泳动速度和尾鞭打频率呈密切相关(r=0.83)。展开更多
基金Supported by Research Fund for the Control of Infectious Diseases and Research Grant Committee of Hong Kong Government
文摘AIM: To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen. METHODS: Hepatitis B viral DNA was isolated from HBV carriers' semen and sera using phenol extraction method and QIAamp DNA blood mini kit (Qiagen, Germany). HBV DNA was detected by conventional PCR and quantified by TaqMan technology-based real-time PCR (quantitative polymerase chain reaction (qPCR)). The detection threshold was 200 copies of HBV DNA for conventional PCR and 10 copies of HBV DNA for real time PCR per reaction. RESULTS: Both methods of phenol extraction and QIAamp DNA blood mini kit were suitable for isolating HBV DNA from semen. The value of the detection thresholds was 500 copies of HBV DNA per mL in the semen. The viral loads were 7.5×10^7 and 1.67×10^7 copies of HBV DNA per mL in two HBV infected patients' sera, while 2.14×10^5 and 3.02×10^5 copies of HBV DNA per mL in the semen. CONCLUSION: Real-time PCR is a more sensitive and accurate method to detect and quantify HBV DNA in the semen.
文摘本文报告用显微摄像监视系统测定107例(生育组30例、不育组77例)精液的精子泳运速度,生育组25.09±4.36μm/s;不育组18.02±4.62μm/s,两组 t 检验具显著差异(t=6.71,P<0.01)。用相差显微镜测定精子尾部摆动(鞭打)频率,生育组7.85±1.29Hz;不育组6.09±11.36Hz,两组 t 检验具显著差异(t=5.88,P<0.01)。精子泳动速度和尾鞭打频率呈密切相关(r=0.83)。