Arabis mosaic virus(ArMV)had been found in the Gladiolus hybridus imported from the Netherlands by DAS-ELISA.Two pairs of primers were designed according to the conserved region of the coat protein(CP) gene sequence o...Arabis mosaic virus(ArMV)had been found in the Gladiolus hybridus imported from the Netherlands by DAS-ELISA.Two pairs of primers were designed according to the conserved region of the coat protein(CP) gene sequence of Arabis mosaic virus(ArMV),and two amplified bands with expected sizes of 750 bp and 250 bp were obtained by reverse transcription polymerase chain reaction(RT-PCR).The sensitivity of nested PCR was higher than that of single RT-PCR.Analysis of the partial sequenced CP gene showed that the isolate was closed to ArMV with similarity of 99.0%-100%.All the results demonstrated that the isolated virus was ArMV.展开更多
从牡丹皮中提纯的丹皮酚结晶(0.6 mg.mL-1)与烟草花叶病毒(Tobacco m osaic virus,TMV)混合10 m in后对TMV枯斑抑制率达80%以上,说明丹皮酚对叶圆片中的病毒复制具有抑制作用;电镜下观察丹皮酚与TMV混合对粒体形态没有影响;丹皮酚与TMV...从牡丹皮中提纯的丹皮酚结晶(0.6 mg.mL-1)与烟草花叶病毒(Tobacco m osaic virus,TMV)混合10 m in后对TMV枯斑抑制率达80%以上,说明丹皮酚对叶圆片中的病毒复制具有抑制作用;电镜下观察丹皮酚与TMV混合对粒体形态没有影响;丹皮酚与TMV混合后透析病毒可恢复侵染力,表明丹皮酚对病毒没有直接毒害作用;用丹皮酚喷施叶面不影响病毒侵染;低含量丹皮酚可阻碍TMV-CP的体外聚合,对TMV-RNA无体外抑制侵染作用.据此推测,丹皮酚对TMV的抑制作用可能主要是通过其与TMV-CP结合而影响病毒粒体对叶片表面侵染位点的识别,或影响植株体内TMV-CP亚基的体内聚合.展开更多
文摘Arabis mosaic virus(ArMV)had been found in the Gladiolus hybridus imported from the Netherlands by DAS-ELISA.Two pairs of primers were designed according to the conserved region of the coat protein(CP) gene sequence of Arabis mosaic virus(ArMV),and two amplified bands with expected sizes of 750 bp and 250 bp were obtained by reverse transcription polymerase chain reaction(RT-PCR).The sensitivity of nested PCR was higher than that of single RT-PCR.Analysis of the partial sequenced CP gene showed that the isolate was closed to ArMV with similarity of 99.0%-100%.All the results demonstrated that the isolated virus was ArMV.
文摘从牡丹皮中提纯的丹皮酚结晶(0.6 mg.mL-1)与烟草花叶病毒(Tobacco m osaic virus,TMV)混合10 m in后对TMV枯斑抑制率达80%以上,说明丹皮酚对叶圆片中的病毒复制具有抑制作用;电镜下观察丹皮酚与TMV混合对粒体形态没有影响;丹皮酚与TMV混合后透析病毒可恢复侵染力,表明丹皮酚对病毒没有直接毒害作用;用丹皮酚喷施叶面不影响病毒侵染;低含量丹皮酚可阻碍TMV-CP的体外聚合,对TMV-RNA无体外抑制侵染作用.据此推测,丹皮酚对TMV的抑制作用可能主要是通过其与TMV-CP结合而影响病毒粒体对叶片表面侵染位点的识别,或影响植株体内TMV-CP亚基的体内聚合.
