Peach brown rot, caused by Monilinia fructicola, is one of the most serious peach diseases. A strain belonging to the Actinomycetales, named Streptomyces blastmyceticus JZB130180, was found to have a strong inhib-itor...Peach brown rot, caused by Monilinia fructicola, is one of the most serious peach diseases. A strain belonging to the Actinomycetales, named Streptomyces blastmyceticus JZB130180, was found to have a strong inhib-itory effect on M. fructicola in confrontation culture. Following the inoculation of peaches in vitro, it was revealed that the fermentation broth of S. blastmyceticus JZB130180 had a significant inhibitory effect on disease development by M. fructicola. The fermentation broth of S. blastmyceticus JZB130180 had an EC50(concentration for 50% of maximal effect) of 38.3 μg/mL against M. fructicola, as determined in an indoor toxicity test. Analysis of the physicochemical properties of the fermentation broth revealed that it was tolerant of acid and alkaline conditions, temperature, and ultraviolet radiation. In addition, chitinase, cellulase, and protease were also found to be secreted by the strain. The results of this study suggest that S. blastmyceticus JZB130180 may be used for the biocontrol of peach brown rot.展开更多
Viruses cause many severe diseases in both plants and animals,urging us to explore new antiviral strategies.In their natural reservoirs,viruses live and replicate while causing mild or no symptoms.Some animals,such as...Viruses cause many severe diseases in both plants and animals,urging us to explore new antiviral strategies.In their natural reservoirs,viruses live and replicate while causing mild or no symptoms.Some animals,such as bats,are the predicted natural reservoir of multiple viruses,indicating that they possess broad-spectrum antiviral capabilities.Mechanisms of host defenses against viruses are generally studied independently in plants and animals.In this article,we speculate that some antiviral strategies of natural reservoirs are conserved between kingdoms.To verify this hypothesis,we created null mutants of 10-formyltetrahydrofolate synthetase(AtTHFS),an Arabidopsis thaliana homologue of methylenetetrahydrofolate dehydrogenase,cyclohydrolase and formyltetrahydrofolate synthetase 1(MTHFD1),which encodes a positive regulator of viral replication in bats.We found that disruption of AtTHFS enhanced plant resistance to three different types of plant viruses,including the tomato spotted wilt virus(TSWV),the cucumber mosaic virus(CMV)and the beet severe curly top virus(BSCTV).These results demonstrate a novel antiviral strategy for plant breeding.We further discuss the approaches used to identify and study natural reservoirs of plant viruses,especially those hosting many viruses,and highlight the possibility of discovering new antiviral strategies from them for plant molecular breeding and antiviral therapy.展开更多
基金Project supported by the Beijing Municipal Science and Technology Plan Projects(No.D151100003915002)the Special Project for Innovation Ability Construction from the Beijing Academy of Agriculture and Forestry Sciences(No.KJCX20170415)+2 种基金the Beijing Agriculture Innovation Consortium(No.BAIC07-2018)the Science and Technology Innovation Ability Construction Fund from the Beijing Academy of Agriculture and Forestry Sciences(No.KJCX20170107)the Science and Technology Innovation Team of the Beijing Academy of Agriculture and Forestry Sciences(No.JNKST201607),China
文摘Peach brown rot, caused by Monilinia fructicola, is one of the most serious peach diseases. A strain belonging to the Actinomycetales, named Streptomyces blastmyceticus JZB130180, was found to have a strong inhib-itory effect on M. fructicola in confrontation culture. Following the inoculation of peaches in vitro, it was revealed that the fermentation broth of S. blastmyceticus JZB130180 had a significant inhibitory effect on disease development by M. fructicola. The fermentation broth of S. blastmyceticus JZB130180 had an EC50(concentration for 50% of maximal effect) of 38.3 μg/mL against M. fructicola, as determined in an indoor toxicity test. Analysis of the physicochemical properties of the fermentation broth revealed that it was tolerant of acid and alkaline conditions, temperature, and ultraviolet radiation. In addition, chitinase, cellulase, and protease were also found to be secreted by the strain. The results of this study suggest that S. blastmyceticus JZB130180 may be used for the biocontrol of peach brown rot.
基金supported by the Taishan Scholar Foundation of Shandong Province (tsqn202103160)the Excellent Youth Foundation of Shandong Scientific Committee (ZR202103010168) for H.Z.+2 种基金the Natural Science Foundation of Shandong Province (ZR2020MC026) for L.L.Postdoctoral Applied Research Project of Qingdao (61200070311121) for W.L.China Postdoctoral Science Foundation (2021T140017) for W.P
文摘Viruses cause many severe diseases in both plants and animals,urging us to explore new antiviral strategies.In their natural reservoirs,viruses live and replicate while causing mild or no symptoms.Some animals,such as bats,are the predicted natural reservoir of multiple viruses,indicating that they possess broad-spectrum antiviral capabilities.Mechanisms of host defenses against viruses are generally studied independently in plants and animals.In this article,we speculate that some antiviral strategies of natural reservoirs are conserved between kingdoms.To verify this hypothesis,we created null mutants of 10-formyltetrahydrofolate synthetase(AtTHFS),an Arabidopsis thaliana homologue of methylenetetrahydrofolate dehydrogenase,cyclohydrolase and formyltetrahydrofolate synthetase 1(MTHFD1),which encodes a positive regulator of viral replication in bats.We found that disruption of AtTHFS enhanced plant resistance to three different types of plant viruses,including the tomato spotted wilt virus(TSWV),the cucumber mosaic virus(CMV)and the beet severe curly top virus(BSCTV).These results demonstrate a novel antiviral strategy for plant breeding.We further discuss the approaches used to identify and study natural reservoirs of plant viruses,especially those hosting many viruses,and highlight the possibility of discovering new antiviral strategies from them for plant molecular breeding and antiviral therapy.
