Rheumatoid Arthritis (RA) which is very difficult to be treated belons to “Bi Zheng” (impediment pattern) in TCM.As the cause of RA can lead to blood stasis in network vessels and blood stasis can occur during the c...Rheumatoid Arthritis (RA) which is very difficult to be treated belons to “Bi Zheng” (impediment pattern) in TCM.As the cause of RA can lead to blood stasis in network vessels and blood stasis can occur during the course of RA,there are great relations between RA and blood stasis of network vessels.The main clinical symptoms of blood stasis syndrome of RA are pain,swelling,local node and ecchymoisis.The blood stasis that runs through the course of RA is both the cause and pathomachanism of RA.展开更多
目的:观察粪菌移植(fecal microbiota transplantation,FMT)、粪菌移植联合果胶(fecal microbiota transplantation combine with pectin,FMTP)治疗对葡聚糖硫酸钠(dextran sodium sulphate,DSS)诱导结肠炎小鼠体重、DAI(disease activi...目的:观察粪菌移植(fecal microbiota transplantation,FMT)、粪菌移植联合果胶(fecal microbiota transplantation combine with pectin,FMTP)治疗对葡聚糖硫酸钠(dextran sodium sulphate,DSS)诱导结肠炎小鼠体重、DAI(disease activity index)指数、血清学、组织病理学等的影响以及结肠黏膜固有层巨噬细胞M1/M2的变化情况,探究粪菌移植治疗溃疡性结肠炎的可能机制。方法:C57BL/6小鼠予以3.0%DSS喂养7 d建造结肠炎模型后按比例随机分为DSS-FMT组(结肠炎模型组,6只)、DSS+FMT组(粪菌移植组,6只)、DSS+FMTP组(粪菌移植联合果胶组,6只),NC组(正常对照组,6只)予以蒸馏水喂养。第8~14 d予以DSS+FMT组、DSS+FMTP组粪菌滤液灌肠处理,0.15 m L/次,隔日1次,共4次;DSS+FMTP组同时予以5%果胶灌胃,0.2 m L/次,1次/日,总共7次。观察各组小鼠体重、疾病活动指数(DAI)、结肠长度、组织病理学、血清学、肠黏膜巨噬细胞M1/M2变化等情况。结果:DSS-FMT组分别与DSS+FMT组、DSS+FMTP组在第10 d体重(16.03+0.23 g vs 17.05±0.70 g、17.45+0.50 g)差异具有统计学意义(P<0.05);第12 d,DSS-FMT组DAI(3.00+0.63)分别与DSS+FMT组(2.33+0.52)、DSS+FMTP组(1.67+0.52)相比均明显升高(P<0.05);DSS-FMT组结肠长度(5.78+0.38)cm相较于DSS+FMT组(6.69+0.50)cm和DSS+FMTP组(7.03±0.47)cm明显缩短(P<0.05);同时,经粪菌移植干预后,与DSS-FMT组相比可以降低血清炎性细胞因子IL-6(53.28+4.40 pg/m L vs 75.35+6.78 pg/m L)、TNF-á(221.76+15.31 ng/L vs 273.10+21.20ng/L),升高抗炎因子IL-4水平(37.19+7.86 pg/m L vs 16.08+12.33 pg/m L)(P<0.05),且可以改变肠黏膜巨噬细胞M1/M2分布、比例。结论:粪菌移植及粪菌移植联合果胶治疗可以改善结肠炎小鼠一般情况、改变结肠黏膜中巨噬细胞M1/M2比例、分布;其缓解结肠炎小鼠肠道炎症可能与促进巨噬细胞M2极化有关。展开更多
文摘Rheumatoid Arthritis (RA) which is very difficult to be treated belons to “Bi Zheng” (impediment pattern) in TCM.As the cause of RA can lead to blood stasis in network vessels and blood stasis can occur during the course of RA,there are great relations between RA and blood stasis of network vessels.The main clinical symptoms of blood stasis syndrome of RA are pain,swelling,local node and ecchymoisis.The blood stasis that runs through the course of RA is both the cause and pathomachanism of RA.
文摘目的:观察粪菌移植(fecal microbiota transplantation,FMT)、粪菌移植联合果胶(fecal microbiota transplantation combine with pectin,FMTP)治疗对葡聚糖硫酸钠(dextran sodium sulphate,DSS)诱导结肠炎小鼠体重、DAI(disease activity index)指数、血清学、组织病理学等的影响以及结肠黏膜固有层巨噬细胞M1/M2的变化情况,探究粪菌移植治疗溃疡性结肠炎的可能机制。方法:C57BL/6小鼠予以3.0%DSS喂养7 d建造结肠炎模型后按比例随机分为DSS-FMT组(结肠炎模型组,6只)、DSS+FMT组(粪菌移植组,6只)、DSS+FMTP组(粪菌移植联合果胶组,6只),NC组(正常对照组,6只)予以蒸馏水喂养。第8~14 d予以DSS+FMT组、DSS+FMTP组粪菌滤液灌肠处理,0.15 m L/次,隔日1次,共4次;DSS+FMTP组同时予以5%果胶灌胃,0.2 m L/次,1次/日,总共7次。观察各组小鼠体重、疾病活动指数(DAI)、结肠长度、组织病理学、血清学、肠黏膜巨噬细胞M1/M2变化等情况。结果:DSS-FMT组分别与DSS+FMT组、DSS+FMTP组在第10 d体重(16.03+0.23 g vs 17.05±0.70 g、17.45+0.50 g)差异具有统计学意义(P<0.05);第12 d,DSS-FMT组DAI(3.00+0.63)分别与DSS+FMT组(2.33+0.52)、DSS+FMTP组(1.67+0.52)相比均明显升高(P<0.05);DSS-FMT组结肠长度(5.78+0.38)cm相较于DSS+FMT组(6.69+0.50)cm和DSS+FMTP组(7.03±0.47)cm明显缩短(P<0.05);同时,经粪菌移植干预后,与DSS-FMT组相比可以降低血清炎性细胞因子IL-6(53.28+4.40 pg/m L vs 75.35+6.78 pg/m L)、TNF-á(221.76+15.31 ng/L vs 273.10+21.20ng/L),升高抗炎因子IL-4水平(37.19+7.86 pg/m L vs 16.08+12.33 pg/m L)(P<0.05),且可以改变肠黏膜巨噬细胞M1/M2分布、比例。结论:粪菌移植及粪菌移植联合果胶治疗可以改善结肠炎小鼠一般情况、改变结肠黏膜中巨噬细胞M1/M2比例、分布;其缓解结肠炎小鼠肠道炎症可能与促进巨噬细胞M2极化有关。
