摘要
自由基研究是开展自由基化学、生物学及医学研究的重要途径。由于自由基活性高、半衰期短,其研究方法呈现多样化的特点。在自由基研究方法中,根据检测仪器的差异较常见的研究方法有电子自旋共振与自旋捕获技术、化学发光法、荧光法、比色法、分光光度法、色谱法和脉冲辐解技术等。由于不同的仪器其检测对象、范围及精度都存在差异,导致研究方法具有不同的特点,因此在众多的实验方案中,根据仪器及研究对象的特点选择合适及合理的研究方法是必要的。
The researching of free radical is basilic researchful approach for free radical's chemistry, biochemistry and medical chemistry. Usually free radical has better activity and shorter half life, hence there have many methods for us to study free radical. In these methods, according to different apparatus, they can be seen as electron spin resonance, chemiluminescence, fluorescence, spectrophotometry, chromatography and radiolysis, etc. Because different apparatus has different detecting object, area and precision, each kind of methods has it's own specialty. Hence it is necessary for us to find and select a right way to study.
出处
《重庆大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2004年第2期73-78,共6页
Journal of Chongqing University
