摘要
尽管酵母人工染色体 (Yeast artificial chrom osome,YAC)存在着克隆效率低、嵌合体出现率高、部分克隆不稳定和缺失等弊端 ,但由于它容纳高分子量 DNA的优点其它载体无法替代 ,而成为人类和动物基因组研究中最为主要的研究工具之一。因此 ,利用 YAC载体系统构建我国濒危畜禽品种的基因组文库 ,对于在分子水平保存国家重要的基因资源和深入的分子生物学研究等方面 ,均具有非常重要的意义。
There were some disadvantages on yeast artificial chromosome (YAC) including low cloning efficiency, high incidence of chimeric clones, instability and deletions of insert DNA, but YAC took large DNA inserts that was not able to replace by the other vector, which made YAC to be one of the important tool in study of human and animal genome. Therefore, it has very important significance to construct the genomic library of endangered domestic animal genetics resources by YAC for protection important resources of gene in molecular level and further study in molecular biology.
出处
《中国农业科技导报》
CAS
CSCD
2003年第4期77-79,共3页
Journal of Agricultural Science and Technology
基金
国家自然科学基金 ( 3 0 10 0 13 2 )
基础性工作重大专项 ( 2 0 0 1DEA10 0 0 6)资助
关键词
濒危畜禽品种
基因组文库
酵母人工染色体
种质资源
YAC
yeast artificial chromosome (YAC)
endangered domestic animal genetics resources
the genomic library
