摘要
[目的]对安徽某土鸡场送检的疑似肿瘤病的病料进行鉴定。[方法]采集病死鸡的肝脏,提取病毒DNA,根据Genbank已登录的基因序列设计引物,通过PCR检测,对扩增出的ALV env与MDV meq基因序列进行测序和序列分析。[结果]检测到禽白血病和马立克目的条带,序列比对结果发现,禽白血病病毒为J亚群,马立克为野毒强毒株。[结论]研究结果为了解该地区禽白血病和马立克混合感染情况提供了参考数据。
[Objective]To identify samples of suspected tumor disease from a local chicken farm in Anhui Province.[Method]The chicken liver was collected and the virus DNA was extracted.The primers were designed according to the sequence of ALV and MDV that GenBank had registered.The ALV env and MDV meq gene were detected by PCR.The amplified sequence was sequenced and analyzed by evolutionary tree and nucleotide homology.[Result]Detected bands of ALV and MD,and sequence alignment results showed that ALV belongs to subgroup J,while MD is a strong wild virus strain.[Conclusion]This study provided reference data for understanding the mixed infection situation of ALV and MDV in the area.
作者
胡晓苗
殷冬冬
沈学怀
潘孝成
戴银
赵瑞宏
周学利
侯宏艳
尹磊
刘亚云
HU Xiao-miao;YIN Dong-dong;SHEN Xue-huai(Institute of Animal Husbandry and Veterinary Medicine,Anhui Academy of Agricultural Sciences/Livestock and Poultry Epidemic Diseases Research Center of Anhui Province/Anhui Province Key Laboratory of Livestock and Poultry Product Safety Engineering,Hefei,Anhui 230031)
出处
《安徽农业科学》
CAS
2024年第18期58-60,66,共4页
Journal of Anhui Agricultural Sciences
基金
安徽省重点研究与开发计划项目(202204c06020039)
安徽省家禽产业技术体系(AHCYJSTX-06)
安徽农科院平台项目(2023YL013)
国家蛋鸡产业技术体系合肥综合试验站(CARS-40-S10)。
关键词
禽白血病
马立克
PCR
序列分析
Avian leukosis(AL)
Marek’s disease(MD)
PCR
Sequence analysis
