摘要
植物蛋白纤维由于其独特的结构和极高的纵横比可作为良好的疏水性生物活性物质的递送载体。为了提高玉米醇溶蛋白在酸热诱导下的纤维化程度,该研究采用低温等离子体技术辅助完成玉米醇溶蛋白自组装纤维化,探究诱导过程中各因素对玉米醇溶蛋白纤维化程度的影响机制,并通过单因素和响应面试验获得最优纤维化工艺。硫黄素T(ThT)荧光强度结果显示,采用等离子体辅助酸热诱导可以有效提高玉米醇溶蛋白的纤维化程度。通过单因素和响应面优化试验获得制备纤维化玉米醇溶蛋白的优化工艺为:等离子体处理功率64 W,处理时间61 s,加热时间为10 h,加热温度70℃,蛋白质量浓度30 mg/mL。在此条件下纤维化玉米醇溶蛋白ThT荧光强度可达2272±23,显著高于未经等离子体处理的纤维化玉米醇溶蛋白(1239±19)(P<0.05)。对傅里叶变换红外光谱中酰胺I区结果分析表明,等离子体处理使玉米醇溶白中β-折叠结构增加,α-螺旋结构减少。透射电子显微镜观察到玉米醇溶蛋白经过纤维化后颗粒粒径明显下降,其纤维结构是由球状蛋白颗粒沿水平方向线性聚集而成,采用等离子体辅助可诱导形成更多的玉米醇溶蛋白纤维体结构。研究结果可为疏水性植物蛋白实现高效自组装纤维化提供参考。
Protein fibers are characterized by the self-assembled linear aggregates of proteins or peptides.Furthermore,the protein fibers can be expected to serve as an effective delivery carrier for water-insoluble bioactive compounds in food systems.Specifically,a better carrier can be generated for the delivery of hydrophobic bioactive substances,due mainly to its unique structure and high aspect ratio.Conventionally,protein fibrous aggregates are usually formed by proteins rich inβ-sheet structure under the conditions of far below isoelectric point pH value and low ionic strength using long-term heating.However,the low preparation efficiency and high energy consumption have greatly limited to select raw materials for protein fibers.The traditional acid-thermal method has also been used to obtain the zein hydrophobic plant proteins at the low conversion rate,due to its special solubility and secondary structure.The application of such protein fiber is still lacking in the field of protein fibrosis.Among them,zein is one of the most important by-products in the process of industrial extraction of corn starch.The self-assembly properties of zein can have promising potential to be an ideal substrate for the fabrication of nano-biocomposite delivery systems in food and nutritional applications.Alternatively,the plasmas were the quasi-neutral particle systems of semigas and semi-fluid mixtures with high active moieties.The green,emerging,and non-thermal plasma technology has also great application potential in the food industry.This study aims to improve the fiber conversion rate of zein using plasma technology.An assessment treatment was innovatively used to modify the zein before acid-heat induction.A systematic investigation was then implemented to explore the influence of different process parameters during acid-heat induction on the degree of fibrosis of zein after plasma treatment.Single factor and response surface experiments were conducted to obtain the optimal condition of the fibrosis process in the plasma treatme
作者
郭鹏
景成童
韩思琦
涂安
王亚潇
董爽
GUO Peng;JING Chengtong;HAN Siqi;TU An;WANG Yaxiao;DONG Shuang(School of Agriculture Engineering and Food Science,Shandong University of Technology,Zibo 255000,China;Shandong Bandaojing Shares Co,Ltd,Zibo 256399,China)
出处
《农业工程学报》
EI
CAS
CSCD
北大核心
2024年第9期281-288,共8页
Transactions of the Chinese Society of Agricultural Engineering
基金
山东省自然科学基金面上项目(ZR2023MC022)
国家自然科学基金青年项目(31901607)。
