摘要
目的 观察“手十二井穴”放血预处理对大鼠急性低氧肺组织损伤模型血气相关指标、低氧诱导因子(HIF)-1α及血管内皮细胞生长因子(VEGF)表达的影响,探讨“手十二井穴”放血预处理对急性低氧肺组织损伤的影响。方法 将72只雄性SD大鼠随机分为对照组8只、低氧(模型)组32只、井穴放血预处理(放血)组32只,模型组和放血组又分为6、24、48、72 h 4个时间段亚组,每组各8只。采用实验动物低氧模拟舱模拟15%O_(2)浓度,制备大鼠急性低氧肺组织损伤模型。放血组按“少商”“商阳”“中冲”“关冲”“少冲”“少泽”的顺序,每天1次,共5 d。用手持式血气分析仪检测肺动脉血气氧分压(PaO_(2))、血氧饱和度(SaO_(2))、酸碱度(pH)、二氧化碳分压(PaCO_(2))等相关指标,采用Western印迹法检测HIF-1α表达,酶联免疫吸附试验(ELISA)法检测VEGF含量。结果 与对照组比较,模型组各时间段PaO_(2)、SaO_(2)明显降低,HIF-1α和VEGF表达水平显著升高(P<0.01)。与同时间段模型组比较,放血组PaO_(2)和SaO_(2)均出现升高,PaO_(2)在6、48 h时有统计学意义,SaO_(2)值在6、24、48 h时有统计学意义(P<0.05,P<0.01),HIF-1α表达在各时段明显下调,VEGF含量在24、48、72 h时明显下降(P<0.05,P<0.01)。但放血组PaO_(2)各时间段和SaO_(2)(6、24 h)仍低于对照组,HIF-1α(6、48、72 h)和VEGF各时间段表达水平仍高于对照组,差异有统计学意义(P<0.05,P<0.01)。结论 “手十二井穴”放血预处理在一定时间和程度上可改善大鼠急性低氧(15%O_(2),85%N_(2))肺组织损伤模型的缺氧情况,其可能的作用与升高动脉血PaO_(2)、SaO_(2)及下调HIF-1α表达、降低VEGF含量有关,从而有利于模型大鼠适应急性低氧环境。
Objective To observe the effects of bloodletting pretreatment at Twelve well points of hands on blood gas indexes,the expression levels of hypoxic inducible factor(HIF)-1αand vascular endothelial growth factor(VEGF)of rats model with acute hypoxic lung tissue injury,so as to investigate its effects on acute hypoxic lung tissue injury.Methods 72 male SD rats were randomly divided into control group of 8 rats,hypoxic(model)group of 32 rats,and well point bloodletting pretreatment(bloodletting)group of 32 rats.The model group and bloodletting group were divided into 4 subgroups at different time of 6,24,48 and 72 h,with 8 rats in each subgroup.The acute hypoxic lung tissue injury model of rats was established by simulating the concentration of 15%O_(2) in the hypoxic simulation chamber of experimental animals.Bloodletting group was intervened in the order of"Shaoshang""Shangyang""Zhongchong""Guanchong""Shaochong"and"Shaoze",once a day for 5 d.The hematology analyzer was used to detect the blood gas indexes of pulmonary arterial oxygen tension(PaO_(2)),arterial oxygen saturation(SaO_(2)),potential hydrogen(pH)and partial pressure of carbon dioxide(PaCO_(2)).The expression of HIF-1αwas detected by Western blot,and the content of VEGF was detected by enzyme linked immunosorbent assay(ELISA).Results Compared with the control group,PaO_(2) and SaO_(2) were significantly decreased and HIF-1αand VEGF expression levels were significantly increased in the model group at the corresponding periods(P<0.01).Compared with the model group at the same time point,PaO_(2) and SaO_(2) in the bloodletting group were increased,PaO_(2) was statistically significant at 6 and 48 h and SaO_(2) was statistically significant at 6,24 and 48 h(P<0.05,P<0.01),the expression level of HIF-1αwas significantly decreased at the corresponding period and the content of VEGF was significantly decreased at 24,48 and 72 h(P<0.05,P<0.01).But PaO_(2) at the corresponding period and SaO_(2) at 6 and 24 h in the bloodletting group were lower than those in
出处
《中国老年学杂志》
CAS
北大核心
2024年第7期1647-1651,共5页
Chinese Journal of Gerontology
基金
国家自然科学基金(81760890)。
