摘要
为筛选副猪嗜血杆菌(Haemophilus parasuis,HPS)4型攻毒菌株,对8株HPS进行血清型鉴定,并绘制细菌生长曲线;用豚鼠攻毒实验对其中5个菌株进行毒力测定,同时记录发病豚鼠的临床症状和剖检病变。结果显示8个菌株均为HPS4型,确定了候选菌株的最佳扩大培养时间为8~10h,筛选出毒力最强的CVCC4355。本研究确定CVCC4355可作为HPS4型疫苗产品效力检验的攻毒菌株。
In order to screen the challenge strains of Hemophilus parasuis(HPS)serotype 4,8 strains were identified by serotype identification and the bacterial growth curve was drawn.Five strains were tested by guinea pig challenge test,and the clinical symptoms and pathological changes of infected guinea pigs were recorded.The results showed that all the 8 strains were HPS serotype 4,and the optimal extended culture time of candidate strains was determined to be 8~10 hours,and CVCC4355 with the strongest virulence was selected.In this study,CVCC4355 strain was identified as a challenge strain for testing the efficacy of HPS serotype 4vaccineproducts.
作者
刘元杰
王秀丽
李建
辛凌翔
朱良全
张一帜
姚文生
刘燕
LIU Yuan-jie;WANG Xiu-li;LI Jian;XIN Ling-xiang;ZHU Liang-quan;ZHANG Yi-zhi;YAO Wen-sheng;LIU Yan(China Institute of Veterinary Drug Control,Beijing 100081,China)
出处
《中国兽药杂志》
2024年第1期6-12,共7页
Chinese Journal of Veterinary Drug
基金
国家重点研发计划(2022YFD1800903)
中国兽医药品监察所兽药行业公益性重点专项(GY202102)。
关键词
副猪嗜血杆菌4型
筛选
毒力测定
Haemophilus parasuis serotype 4
screen
virulence test
