摘要
目的 研究阿魏属植物臭阿魏Ferula teterrima的化学成分及其抗肿瘤活性。方法 采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱及半制备高效液相色谱等色谱方法分离纯化得到单体化合物,利用MS、1H-NMR、13C-NMR、DEPT、2D NMR等波谱学方法鉴定化合物的结构。采用计算电子圆二色谱法(ECD)判定新化合物的绝对构型。同时以人肝癌Hep G2细胞株为筛选模型对分离得到的化合物进行抗肿瘤活性测试。结果 从臭阿魏中分离得到了9个化合物,分别鉴定为臭阿魏脂素A(1)、镰叶芹二醇(2)、阿魏酸(3)、阿魏酸松柏酯(4)、badrakemin acetate(5)、fekolone(6)、mogoltadone(7)、香草醛(8)、γ-甲氧基异丁香酚(9)。化合物1为降木脂素类成分,2为聚炔类成分,5~7为倍半萜香豆素类成分,3、4为苯丙素类成分。结论 化合物1为新化合物,化合物2、4和9为首次从阿魏属中分离得到,化合物3、6~8为首次从该植物中得到。化合物4、6和7表现出潜在的抗肿瘤活性,半数抑制浓度(median inhibition concentration,IC50)分别为11.09、17.95和23.06μmol/L。
Objective To study the chemical constituents from Ferula teterrima and their antitumor activities.Methods The chemical constituents were separated and purified by silica gel,Sephadex LH-20,semi-prepared HPLC and other chromatography techniques.The structures of monomer compounds were determined by MS,1H-NMR,13C-NMR,DEPT,2D NMR and other spectroscopic methods.The absolute configuration of new compound was determined by ECD.The cytotoxic activity of isolated compounds on human liver cancer(HepG2)cell line was determined.Results Nine compounds(1—9)were isolated,and were identified as teterrimanol A(1),falcarindiol(2),ferulic acid(3),coniferyl ferulate(4),badrakemin acetate(5),fekolone(6),mogoltadone(7),vanillin(8),2-methoxy-4-(3-methoxy-1-propenyl)-phenol(9).Compounds 1 were lignans,2 were polyacetylene,5—7 were sesquiterpene coumarins,and 3 and 4 were phenylpropanes Conclusion Compound 1 is a new compound;Compounds 2,4 and 9 are isolated from the genus Ferula for the first time;Compounds 3,6—8 are isolated from this plant for the first time.Compounds 4,6 and 7 showed potential antitumor activity with 50%inhibitory concentration(IC50)values of 11.09,17.95 and 23.06μmol/L,respectively.
作者
张旭
李敏
王月
陈倩
刘敬勃
李韶勇
唐生安
ZHANG Xu;LI Min;WANG Yue;CHEN Qian;LIU Jing-bo;LI Shao-yong;TANG Sheng-an(Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics(Theranostics),School of Pharmacy,Tianjin Medical University,Tianjin 300070,China)
出处
《中草药》
CAS
CSCD
北大核心
2023年第8期2373-2378,共6页
Chinese Traditional and Herbal Drugs
基金
天津市教委科研计划项目(2019ZD031)。
