摘要
以滇中植烟土壤为研究对象,设置不同厨余垃圾堆肥(基肥)施用量:不施肥对照(CK)、低施肥量8 t/hm^(2)(FWL)、中施肥量16 t/hm^(2)(FWM)、高施肥量24 t/hm^(2)(FWH),研究厨余垃圾堆肥施用后土壤酶活性和细菌群落结构的变化特征,为厨余垃圾堆肥农田推广使用提供依据。结果表明:与CK相比,FWH处理可显著提高土壤的pH、有机碳、全氮、全磷、碱解氮、有效磷、脲酶活性、酸性磷酸酶活性、荧光素二乙酸水解酶活性(↑0.39%~89.42%)、土壤细菌OUT数目(↑12.91%)、Chao指数(↑11.20%)、Ace指数(↑11.37%)。变形菌门(Proteobacteria)的相对丰度随施肥量的增加而增加,而酸杆菌门(Acidobacteria)则相反;细菌群落的膜运输和碳水化合物代谢功能均在施肥后显著增强。脲酶活性、酸性磷酸酶活性、pH、全氮、全磷、碱解氮、有效磷指标对土壤细菌的群落组成和多样性均有影响,其中碱解氮和脲酶活性是调控菌群最主要的因子。
In order to investigate the changes of soil enzyme activity and bacterial community structure after fertilizer application and to provide basic data to support the promotion of food waste compost on farm,flue-cured tobacco growing soils in central Yunnan were taken as study object,different application rates of food waste compost(base fertilizer)were designed:no fertilizer control(CK),low fertilizer application rate(8 t/hm^(2),FWL),medium fertilizer application rate(16 t/hm^(2),FWM),and high fertilizer application rate(24 t/hm^(2),FWH).The results show that compared with CK,FWH significantly increases soil pH,the contents of organic carbon,total nitrogen,total phosphorus,alkaline soluble nitrogen and effective phosphorus,the activities of urease,acid phosphatase,fluorescein diacetate hydrolase activity(↑0.39%–89.42%),soil bacterial OUT number(↑12.91%),Chao index(↑11.20%),Ace index(↑11.37%).The relative abundance of Proteobacteria is increased with increasing fertilizer application,while Acidobacteria is decreased.Both membrane transport and carbohydrate metabolism of the bacterial community are significantly enhanced after fertilization.Urease and acid phosphatase activities,pH,total nitrogen,total phosphorus,alkaline nitrogen and effective phosphorus all have effects on the community composition and diversity of soil bacteria,with alkaline nitrogen and urease activity being the most important factors regulating the bacterial community.
作者
张晓花
王克勤
宋娅丽
杨昕
茶联玲
ZHANG Xiaohua;WANG Keqin;SONG Yali;YANG Xin;CHA Lianling(College of Ecology and Environment,Southwest Forestry University,Kunming 650224,China)
出处
《土壤》
CAS
CSCD
北大核心
2023年第2期321-330,共10页
Soils
基金
云南省科技计划项目(202203AC100001-03)
云南省科技厅“三区”人才支持计划项目(90202101)
云南省大学生创新创业训练计划项目(202110677076)
云南省水土保持监测站(玉溪二龙潭小流域水土保持监测)项目(632166)资助。
关键词
厨余垃圾堆肥
细菌群落
酶活性
Food waste composting
Bacterial community
Enzyme activity
