摘要
为了深入探究油白菜对盐胁迫的形态及生理响应机制,本研究以5叶期油白菜幼苗为研究对象,设置对照组和处理组,各处理组分别根施150 g/L、200 g/L和250 g/L的NaCl溶液,待处理组幼苗出现萎蔫表型时,测定不同处理下油白菜的干湿重比,可溶性蛋白含量、丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、叶绿素含量及脯氨酸含量。试验结果表明:随着盐浓度的上升,油白菜的干湿重比值呈先增加后降低的趋势;SOD、POD活性增加,脯氨酸含量均呈上升趋势,而可溶性蛋白、MDA、叶绿素含量呈先下降后上升的趋势。在150 g/L NaCl浓度下,油白菜的鲜重、可溶性蛋白含量、MDA含量、叶绿素含量最小。本试验通过测定油白菜在不同盐浓度下的形态和生理指标变化,为选育油白菜耐盐材料提供了参考依据。
This study explores the morphology and physiological response mechanism of Chinese cabbage to salt stress,with 5-leaf stage Chinese cabbage seedlings taken as the research object.A control group and three treatments were adopted,and each treatment group was root-applied 150 g/L,200 g/L and 250 g/L of NaCl solution respectively.When the seedlings in the treatment group showed a wilted phenotype,seven indexes were determined under different treatments,including the dry-wet weight ratio,soluble protein content,malondialdehyde(MDA)content,superoxide dismutase(SOD)content,and peroxidase(POD)content,chlorophyll content,and proline content.The experimental results showed that with the increase in salt concentration,the dry-wet weight ratio of Chinese cabbage increased first and then decreased,the activity of SOD and POD increased,and the proline content showed an upward trend.In contrast,the soluble protein,MDA,and chlorophyll content showed a decreasing and rising trend.At a concentration of 150 g/L of NaCl,the fresh weight,soluble protein content,MDA content,and chlorophyll content of Chinese cabbage were minimal.In this experiment,the changes in the morphological and physiological index in Chinese cabbage under different salt concentrations were determined,which provided a reference basis for selecting and cultivating salt-tolerant materials for Chinese cabbage.
作者
郭亚宁
艾静
GUO Ya-ning;AI Jing(School of Life Sciences,Yulin University,Yulin 719000,China)
出处
《榆林学院学报》
2023年第2期38-41,共4页
Journal of Yulin University
基金
榆林学院博士科研启动基金(18GK12)。
关键词
盐胁迫
油白菜
生理指标
形态指标
salt stress
Chinese cabbage
morphological index
physiological index
