摘要
为建立同时测定地顶孢霉培养物中腺苷、虫草素和麦角甾醇含量的高效液相色谱法,选择色谱柱为Waters C18柱(4.6 mm×250 mm,5μm),流动相为水-乙腈,梯度洗脱,检测波长270 nm,流速1 mL·min^(-1),柱温30℃,进样量20μL。结果表明:腺苷、虫草素和麦角甾醇在浓度20~640μg·mL-1(X)范围内与峰面积(Y)呈良好的线性关系,腺苷、虫草素和麦角甾醇的回归方程分别为Y=98.53X+586.26(R^(2)=0.9993)、Y=124.16X+277.69(R^(2)=0.9997)、Y=57.652X+107.47(R^(2)=0.9996)。腺苷、虫草素和麦角甾醇的加标回收率分别为95.96%~99.95%、97.43%~99.94%和96.31%~99.95%。该方法准确度高、重复性好、灵敏度高,适用于地顶孢霉培养物中腺苷、虫草素和麦角甾醇含量的测定,为其质量监控提供了技术支撑。
A high performance liquid chromatography(HPLC)method was established for the simultaneous determination of adenosine,cordycepin and ergosterol in Acremoniumterricola culture.Separation was completed in Waters C18 column(4.6 mm×250 mm,5μm).Mobile phase was water-acetonitrile.Detection wavelength was set at 270 nm.The flow rate was 1 mL·min^(-1).The column temperature was controlled at 30℃,and the injection volume was 20μL.The calibration curve was good linear between the ratio of the peak areas(Y)and concentrations(X)of adenosine,cordycepin and ergosterol from 20 to 640 ng·mL-1.The regression equations of adenosine,cordycepin and ergosterol were Y=98.53X+586.26,Y=124.16X+277.69,Y=57.652X+107.47,respectively.The R^(2)values were higher than 0.9993.The average recovery rate of adenosine,cordycepin and ergosterol was 95.96%-99.95%,97.43%-99.94%and 96.31%-99.95%,respectively.This method had characteristics of high accuracy,good repeatability and sensitivity,which was suitable for the determination of adenosine,cordycepin and ergosterolin Acremoniumterricolaculture and provide technical support for its quality control.
作者
刘书余
陈晋元
孙清平
郭亚文
管凡荀
高鹏飞
汤亚云
朱雅丽
谢恺舟
LIU Shuyu;CHEN Jinyuan;SUN Qingping;GUO Yawen;GUAN Fanxun;GAO Pengfei;TANG Yayun;ZHU Yali;XIE Kaizhou(College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China;Joint International Research Laboratory of Agriculture and Agri-Product Safety,Yangzhou University,Yangzhou 225009,China;METDO(Jiangsu)Biotechnology Company Limited,Yangzhou 225106,China)
出处
《扬州大学学报（农业与生命科学版）》
CAS
北大核心
2022年第6期111-117,132,共8页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
国家现代农业产业技术体系专项(CARS-41)
江苏省高校优势学科建设工程项目(PAPD)。
关键词
地顶孢霉培养物
腺苷
虫草素
麦角甾醇
高效液相色谱法
同时测定
Acremoniumterricola culture
adenosine
cordycepin
ergosterol
high performance liquid chromatography(HPLC)
simultaneous determination
