摘要
为了提高分子信标(MB)检测肿瘤细胞内微小核糖核酸(miRNA)的灵敏度和准确性,设计了一种酸响应的生物矿化纳米载体BSA/CaP,可高效负载MB制备MB@BSA/CaP纳米探针,用于人源乳腺肿瘤细胞中miRNA-155的检测。BSA/CaP纳米载体为中空球型结构,负载MB后粒径增大,MB负载量最高达到331.24 nmol/L,包封率为82.81%,可在酸性环境中(pH 5.00)释放MB。MB@BSA/CaP纳米探针更容易被肿瘤细胞摄取,其摄取效率是商用脂质体载体的约5倍,可将更多的MB递送至细胞内与miRNA-155结合,显著增强探针对细胞内miRNA-155的荧光成像检测灵敏度。同时,由于BSA/CaP纳米载体可保护MB不被核酸酶降解,减少了假阳性信号的产生,提高了探针检测的准确性。
In order to improve the sensitivity and accuracy of molecular beacon(MB) in the detection of microRNA(miRNA) in tumor cells, the MB@BSA/CaP nanoprobe was obtained by loading MB on the acid-responsive biomineralized BSA/CaP nanocarrier for the detection of miRNA-155 in human breast tumor cells. The size of the nanocarrier with hollow structure increased after loading MB.The loading capacity and encapsulation efficiency of MB@BSA/CaP were calculated to be 331.24 nmol/L and 82.81%. The nanocarrier could release MB under acidic environment(pH 5.00). The cellular uptake efficiency of the nanoprobe was about 5 times as high as that of commercial liposome carriers. Therefore, more MBs could be delivered by the nanocarrier into cells and bind to miRNA-155, thereby significantly enhancing the detection sensitivity of the probe for intracellular miRNA-155 fluorescence imaging. In addition, the BSA/CaP nanocarrier could protect the MB from being degraded by nucleases, reducing the generation of false positive signals and improving the detection accuracy of probe.
作者
吴玉婷
王彩霞
刘志洪
WU Yuting;WANG Caixia;LIU Zhihong(College of Chemistry and Chemical Engineering,Hubei University/Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials/Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules,Wuhan 430062,Hubei,China;College of Chemistry and Molecular Sciences,Wuhan University,Wuhan 430072,Hubei,China)
出处
《武汉大学学报（理学版）》
CAS
CSCD
北大核心
2022年第4期367-374,共8页
Journal of Wuhan University:Natural Science Edition
基金
国家自然科学基金(22107025)。
