摘要
目的探讨长链非编码RNA 01535(LINC01535)靶向微小RNA(miR)-483-3p对肺癌细胞增殖和凋亡的影响。方法将A549细胞分为对照组(不作干预)、si-NC组(转染si-NC)、si-LINC01535组(转染si-LINC01535)、miR-NC组(转染miR-NC)、miR-483-3p组(转染miR-483-3p)、anti-miR-NC+si-LINC01535组(转染anti-miR-NC+si-LINC01535)、anti-miR-483-3p+si-LINC01535组(转染anti-miR-483-3p+si-LINC01535)。细胞计数试剂盒-8(CCK-8)和流式细胞术用于评估细胞活力和凋亡率。双荧光素酶报告实验分析LINC01535和miR-483-3p的关系。结果对照组、si-NC组、si-LINC01535组A549细胞LINC01535表达水平分别为1.00±0.08,1.02±0.09,0.48±0.04;细胞活力分别为1.10±0.10,1.11±0.08,0.54±0.05;凋亡率分别为(6.63±0.47)%,(6.59±0.40)%,(24.76±1.92)%。si-LINC01535组与对照组、si-NC组比较,上述指标差异均有统计学意义(均P<0.05)。miR-NC组、miR-483-3p组、anti-miR-NC+si-LINC01535组、anti-miR-483-3p+si-LINC01535组A549细胞miR-483-3p表达水平分别为1.00±0.10,2.42±0.15,1.00±0.10,0.46±0.05;细胞活力分别为1.11±0.09,0.58±0.04,0.55±0.05,1.07±0.08;凋亡率分别为(6.54±0.32)%,(21.57±1.65)%,(25.42±1.51)%,(6.79±0.41)%。miR-483-3p组与miR-NC组比较,anti-miR-483-3p+si-LINC01535组与anti-miR-NC+si-LINC01535组比较,上述指标差异均有统计学意义(均P<0.05)。LINC01535靶向调控miR-483-3p表达。结论干扰LINC01535可通过靶向上调miR-483-3p抑制肺癌细胞增殖并诱导其凋亡。
Objective To investigate the effect of long non-coding RNA 01535(LINC01535)on cell proliferation and apoptosis in lung cancer cells by targeting microRNA(miR)-483-3 p.Methods A549 cells were divided into control group(no intervention),si-NC group(transfected with si-NC),si-LINC01535 group(transfected with si-LINC01535),miR-NC group(transfected with miR-NC),miR-483-3 p group(transfected with miR-483-3 p),anti-miR-NC+si-LINC01535 group(transfected with anti-miR-NC+si-LINC01535),anti-miR-483-3 p+si-LINC01535 group(transfected with anti-miR-483-3 p+si-LINC01535).Cell counting kit-8(CCK-8)and flow cytometry were used to assess cell viability and apoptosis rate.The relationship between LINC01535 and mi R-483-3p was analyzed by dual-luciferase reporter assay.Results LINC01535 expression of A549 cells in control group,si-NC group and si-LINC01535 group were1.00±0.08,1.02±0.09,0.48±0.04;cell viability were 1.10±0.10,1.11±0.08,0.54±0.05;the apoptosis rate was(6.63±0.47)%,(6.59±0.40)%,(24.76±1.92)%.Compared with the control group and si-NC group,the the above indexes in si-LINC01535 group were statistically significant(all P<0.05).The expression level of mi R-483-3p in A549 cells in mi R-NC group,mi R-483-3p group,anti-mi R-NC+si-LINC01535group,and anti-mi R-483-3p+si-LINC01535 group were 1.00±0.10,2.42±0.15,1.00±0.10,0.46±0.05;cell viability were 1.11±0.09,0.58±0.04,0.55±0.05,1.07±0.08;apoptosis rate were(6.54±0.32)%,(21.57±1.65)%,(25.42±1.51)%,(6.79±0.41)%.Comparing the mi R-483-3p group with the mi R-NC group,and comparing the anti-mi R-483-3p+si-LINC01535 group with the anti-mi R-NC+si-LINC01535 group,the above indexes were statistically significant(all P<0.05).LINC01535 targets mi R-483-3p expression.Conclusion Interfering with LINC01535 inhibited lung cancer cell proliferation and induced apoptosis by targeting and up-regulating mi R-483-3p.
作者
陈联
陈学诚
CHEN Lian;CHEN Xue-cheng(Department of Respiratory and Critical Car eMedicine,The First Affliated Hospital of Fujian Medical University,Fuzhou 350005,Fujian Province,China;Departmentof General Medicine,Fuzhou Sino-German Orthopaedic Hospital,Fuzhou 350005,Fujian Province,China)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2022年第20期2429-2433,共5页
The Chinese Journal of Clinical Pharmacology
