摘要
为评估饮用水中新型消毒副产物N-亚硝胺对食品饮料行业带来的潜在风险,建立一种基于超高效液相色谱-三重四极杆质谱联用仪同时检测饮用水中9种N-亚硝胺类消毒副产物的方法。样品通过固相萃取,进入超高效液相色谱-质谱检测系统,经RRHD-C18色谱柱分离,以含有0.2%甲酸的10 mmol/L碳酸氢铵溶液和甲醇作为流动相梯度洗脱,采用大气压化学电离正离子多反应监测扫描模式检测。在优化条件下9种N-亚硝胺在一定质量浓度范围内线性关系良好,相关系数大于0.996,方法检出限为0.10~0.33 ng/L,方法的回收率为62.1%~118.2%,相对标准偏差为2.2%~10.0%,分析时间为6 min。与现有方法比较,本方法灵敏度高、检测速度快、适用性强,能满足食品饮料生产中饮用水N-亚硝胺类消毒副产物的检测需求。
In order to evaluate the potential risks posed by N-nitrosamines,a group of emerging disinfection by-products in drinking water,to the food and beverage industry,a method based on ultra-high performance liquid chromatography-triple quadrupole mass spectrometry(UPLC-MS/MS)was developed for the simultaneous determination of nine N-nitrosamine disinfection by-products in drinking water.The samples were extracted by solid phase extraction.The chromatographic separation was accomplished on a RRHD-C18 column with gradient elution using a mobile phase consisting of 10 mmol/L ammonium bicarbonate aqueous solution containing 0.2%formic acid and methanol.The detection was carried out using an atmospheric pressure chemical ionization(APCI)source in a multi-reaction monitoring(MRM)mode.Under optimized conditions,the calibration curves for nine N-nitrosamines were linear in a certain concentration range,with correlation coefficient greater than 0.996,and the detection limit of the developed method was 0.10–0.33 ng/L.The spiked recoveries of the method were 62.1%–118.2%,with relative standard deviation(RSD)of 2.2%–10.0%.The analysis process took 6 min.Compared with the existing methods,this method is very sensitive,fast and highly applicable,and can meet the requirements for the detection of N-nitrosamine disinfection by-products in drinking water for food and beverage production.
作者
孙铭
古其会
张菊梅
郭伟鹏
吴慧清
张友雄
韦献虎
吴清平
SUN Ming;GU Qihui;ZHANG Jumei;GUO Weipeng;WU Huiqing;ZHANG Youxiong;WEI Xianhu;WU Qingping(Guangzhou Institute of Chemistry,Chinese Academy of Sciences,Guangzhou 510650,China;Guangdong Provincial Key Laboratory of Microbial Safety and Health,State Key Laboratory of Applied Microbiology Southern China,Institute of Microbiology,Guangdong Academy of Sciences,Guangzhou 510070,China;University of Chinese Academy of Sciences,Beijing 100049,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2022年第2期310-315,共6页
Food Science
基金
国家自然科学基金青年科学基金项目(31901663)
广东省科技计划项目(2020B121202009)
广东省科学院发展专项(2019GDASYL0103006)。
关键词
超高效液相色谱-三重四极杆质谱
N-亚硝胺类消毒副产物
固相萃取
饮用水
ultra-high performance liquid chromatography-triple quadrupole mass spectrometry
N-nitrosamine disinfection by-products
solid phase extraction
drinking water
